| Synthesis of the foetal protein fetuin by early developing neurons in the immature neocortex. | |
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MedLine Citation:
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PMID: 7683041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of the foetal protein fetuin has previously been demonstrated by immunocytochemistry to be specifically confined to the primordial plexiform layer, the early cortical plate and subplate zone cells in the developing neocortex of a number of species. In order to investigate its origin there, we have applied in situ hybridization in paraffin sections of Bouin's fixed foetal sheep brain, using a short anti-sense oligonucleotide probe. The distribution of fetuin mRNA has been compared with that of the protein by using anti-fetuin antibodies and immunocytochemistry. This allowed us to confirm that fetuin is synthesised initially in cells of the primordial plexiform layer and subsequently cortical plate and subplate cells. On the other hand, cells in the ventricular zone that are fetuin (protein) positive do not contain detectable fetuin mRNA. The time course of fetuin mRNA expression in the developing neocortex follows closely the previously described pattern of fetuin (protein) distribution in the sheep brain, apart from its absence from the ventricular zone where its origin is probably by uptake from cerebrospinal fluid. |
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Authors:
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K M Dziegielewska; M Reader; N Matthews; W M Brown; K Møllgård; N R Saunders |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurocytology Volume: 22 ISSN: 0300-4864 ISO Abbreviation: J. Neurocytol. Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-05-27 Completed Date: 1993-05-27 Revised Date: 2010-08-25 |
Medline Journal Info:
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Nlm Unique ID: 0364620 Medline TA: J Neurocytol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 266-72 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of Tasmania, Hobart, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cerebral Cortex / chemistry, embryology*, metabolism Female Gene Expression Gestational Age Immunoenzyme Techniques In Situ Hybridization Molecular Sequence Data Neurons / metabolism* Oligonucleotide Probes Pregnancy RNA, Messenger / analysis, metabolism Sheep alpha-Fetoproteins / biosynthesis*, genetics |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotide Probes; 0/RNA, Messenger; 0/alpha-Fetoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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