| Synthesis of a fluorescent xanthenic derivative useful for labeling amine residues. | |
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MedLine Citation:
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PMID: 18637685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 2,5-dioxopyrrolidin-1-yl-4-(3-hydroxy-6-oxo-6H-xanthen-9-yl)-3-methylbenzoate has been synthesized as an amine-reactive derivative able to yield stable covalently labeled biopolymers. The new derivative has been used to label polyribocytidilic acid (5'), poly(C), amine residues. TG-II-poly(C) exhibits monoexponential decay at the physiological pH range. In addition, both steady-state fluorescence intensity and fluorescence decay are also sensitive to solution pH. The large decrease in steady-state fluorescence upon hybridization allows it to be used as a nucleic acid probe in a homogeneous assay format. In summary, we report an efficient synthesis to obtain labeled RNA from commercially available materials in excellent yields. |
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Authors:
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Luis Crovetto; Ramon Rios; Jose M Alvarez-Pez; Jose M Paredes; Patricia Lozano-Velez; Carmen del Valle; Eva M Talavera |
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Publication Detail:
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Type: Letter; Research Support, Non-U.S. Gov't Date: 2008-07-19 |
Journal Detail:
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Title: The journal of physical chemistry. B Volume: 112 ISSN: 1520-6106 ISO Abbreviation: J Phys Chem B Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-14 Completed Date: 2008-09-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101157530 Medline TA: J Phys Chem B Country: United States |
Other Details:
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Languages: eng Pagination: 10082-5 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amines
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chemistry* Anions Chemistry, Physical / methods Chromatography / methods Coloring Agents / chemistry* Fluorescein / chemistry Hydrogen-Ion Concentration Kinetics Models, Chemical Models, Theoretical Oligonucleotide Probes / chemistry RNA / chemistry Xanthenes / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Anions; 0/Coloring Agents; 0/Oligonucleotide Probes; 0/Xanthenes; 2321-07-5/Fluorescein; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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