| Synthesis and fluorescence studies of some new fluorophores and their effect on hybridization of oligodeoxyribonucleotides. | |
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MedLine Citation:
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PMID: 17235674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Some novel fluorophores, viz. 6-(6-isobutyrylamino-1,3-dioxo-1H,3H-benzo[de]isoquinolin-2-yl)-hexanoic acid (1), 6-(6-dimethylamino-1,3-dioxo-1H, 3H-benzo[de]isoquinolin-2-yl)-hexanoic acid (2), 6-(6-benzoylamino-1, 3-dioxo-1H, 3H-benzo[de]isoquinolin-2-yl)-hexanoic acid (3), 6-(6-amino-1-oxo-1H, 3H-benzo[de]isoquinolin-2-yl)-hexanoic acid (4) and 6-(6-amino-1H,3H-benzo[de] isoquinolin-2-yl)-hexanoic acid (5) have been designed, synthesized and characterized. Their comparative fluorescence has been studied in different organic solvents and aqueous solutions containing inorganic ions. Out of these, two fluorophores, 1 and 2 have been used for labelling of nucleosides which were finally converted into their phosphoramidites, and used for labelling of oligodeoxyribonucleotides through covalent attachment. These fluorophores after attachment on oligodeoxyribonucleotides showed good fluorescence signals and higher hybridisation affinity than unlabelled oligodeoxyribonucleotides. |
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Authors:
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Shipra Singh; Ramendra K Singh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-18 |
Journal Detail:
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Title: Journal of fluorescence Volume: 17 ISSN: 1053-0509 ISO Abbreviation: J Fluoresc Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-02 Completed Date: 2007-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9201341 Medline TA: J Fluoresc Country: United States |
Other Details:
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Languages: eng Pagination: 139-48 Citation Subset: IM |
Affiliation:
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Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad 211002, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescent Dyes
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chemical synthesis,
chemistry* Hexanoic Acids / chemical synthesis, chemistry* Isoquinolines / chemical synthesis, chemistry* Nucleic Acid Hybridization* Oligodeoxyribonucleotides / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/6-(6-dimethylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; 0/6-(6-isobutyrylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; 0/Fluorescent Dyes; 0/Hexanoic Acids; 0/Isoquinolines; 0/Oligodeoxyribonucleotides |
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