| Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids. | |
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MedLine Citation:
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PMID: 18618490 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN. |
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Authors:
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Fumi Nagatsugi; Ryusuke Nakahara; Kaori Inoue; Shigeki Sasaki |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archiv der Pharmazie Volume: 341 ISSN: 0365-6233 ISO Abbreviation: Arch. Pharm. (Weinheim) Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-10 Completed Date: 2008-10-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0330167 Medline TA: Arch Pharm (Weinheim) Country: Germany |
Other Details:
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Languages: eng Pagination: 562-7 Citation Subset: IM |
Affiliation:
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Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Affinity Luciferases / chemical synthesis*, chemistry* Nucleic Acid Hybridization Oligonucleotides / chemical synthesis*, chemistry* Polyethylene Glycols / chemistry Sequence Analysis, DNA / methods* |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 0/Polyethylene Glycols; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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