Document Detail


Synthesis of dehydrogenation polymers of ferulic acid with high specificity by a purified cell-wall peroxidase from French bean (Phaseolus vulgaris L.).
MedLine Citation:
PMID:  8192663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cationic (pI 8.3) wall-bound peroxidase has been purified to homogeneity from suspension-cultured cells of French bean (Phaseolus vulgaris L.). The enzyme was a glycoprotein and its M(r) was 46,000 as determined by SDS/Page and h.p.l.c. gel filtration. It was localized biochemically to microsomes and the cell wall, and the latter subcellular distribution was confirmed by immunogold techniques. The native enzyme showed absorption maxima at 403, 500 and 640 nm, with an RZ (A405/A280) of 3.3. The peroxidase oxidized guaïacol and natural phenolic acids. By desorption-chemical-ionization mass spectrometry the enzyme was found to oxidize the model compound, ferulic acid, into dehydrodiferulic acid. Kinetics studies indicated an apparent Km of 113.3 +/- 22.9 microM and a Vmax of 144 mumol.min-1.nmol-1 of protein at an H2O2 concentration of 100 microM. In comparison with a second French-bean peroxidase (FBP) and horseradish peroxidase, as a model, it acted with a 6-10-fold higher specificity in this capacity. It is a member of the peroxidase superfamily of bacterial, fungal and plant haem proteins by virtue of its highly conserved amino acid sequence within the proximal and distal haem-binding sites. This is good evidence that this particular FBP may function in constructing covalent cross-linkages in the wall during development and response to pathogens.
Authors:
A Zimmerlin; P Wojtaszek; G P Bolwell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  299 ( Pt 3)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-17     Completed Date:  1994-06-17     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  747-53     Citation Subset:  IM; S    
Affiliation:
Department of Biochemistry, Royal Holloway and Bedford New College, University of London, Egham, Surrey, U.K.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Biopolymers
Cell Wall / enzymology
Chromatography, Gel
Chromatography, High Pressure Liquid
Coumaric Acids / metabolism*
Electrophoresis, Polyacrylamide Gel
Fabaceae / enzymology*
Hydrogenation
Immunohistochemistry
Isoenzymes / isolation & purification,  metabolism
Kinetics
Mass Spectrometry
Molecular Sequence Data
Oxidation-Reduction
Peroxidases / isolation & purification,  metabolism*
Plants, Medicinal*
Sequence Alignment
Chemical
Reg. No./Substance:
0/Biopolymers; 0/Coumaric Acids; 0/Isoenzymes; 1135-24-6/ferulic acid; EC 1.11.1.-/Peroxidases
Comments/Corrections

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