| Synthesis of cyclic di-nucleotidic acids as potential inhibitors targeting diguanylate cyclase. | |
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MedLine Citation:
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PMID: 20797869 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Five analogs of cyclic di-nucleotidic acid including c-di-GMP were synthesized and evaluated for their biological activities on Slr1143, a diguanylate cyclase of Synechocystis sp. Slr1143 was overexpressed from the recombinant plasmid which contained the gene of interest and subsequently purified by affinity chromatography. A new HPLC method capable of separating the compound and product peaks with good resolution was optimized and applied to the analysis of the compounds. Results obtained show that cyclic di-inosinylic acid 1b demonstrates a stronger inhibition on Slr1143 than c-di-GMP and is a potential inhibitor for biofilm formation. |
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Authors:
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Shi Min Ching; Wan Jun Tan; Kim Lee Chua; Yulin Lam |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-04 |
Journal Detail:
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Title: Bioorganic & medicinal chemistry Volume: 18 ISSN: 1464-3391 ISO Abbreviation: Bioorg. Med. Chem. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2010-12-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9413298 Medline TA: Bioorg Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 6657-65 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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antagonists & inhibitors*,
genetics,
metabolism Biofilms / drug effects Chromatography, Affinity Cyclic GMP / analogs & derivatives*, chemical synthesis, chemistry, pharmacology Phosphorus-Oxygen Lyases / antagonists & inhibitors*, genetics, metabolism Purine Nucleotides / chemical synthesis, chemistry*, pharmacology Recombinant Proteins / antagonists & inhibitors, isolation & purification, metabolism Synechocystis / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Purine Nucleotides; 0/Recombinant Proteins; 0/cyclic(3'-5')diinosylic acid; 61093-23-0/bis(3',5')-cyclic diguanylic acid; 7665-99-8/Cyclic GMP; EC 4.6.-/Phosphorus-Oxygen Lyases; EC 4.6.1.-/diguanylate cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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