| Synthesis of cyclic beta-(1,2)-glucans by Rhizobium leguminosarum biovar trifolii TA-1: factors influencing excretion. | |
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MedLine Citation:
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PMID: 1400186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The synthesis of cyclic beta-(1,2)-glucans from UDP-[14C]glucose by a crude membrane preparation and whole cells of Rhizobium leguminosarum bv. trifolii TA-1 was investigated. The crude membrane system needed Mn2+, ATP, and NAD+ for optimal activity. Hardly any difference in biosynthetic activity between membrane fractions of TA-1 cells grown in the presence (200 mM) or absence of NaCl was observed. Whole TA-1 cells grown in the presence of NaCl excreted labeled, neutral cyclic beta-(1,2)-glucan during incubation with added UDP-[14C]glucose. With NaCl-free cultured TA-1 cells, no excretion was observed; however, after these cells were alternately frozen and thawed eight times, they excreted glucans. Glucan formation in vitro and glucan excretion by whole cells were strongly inhibited in the presence of 50 mg of cyclic glucan per ml (about 15 mM), indicating that biosynthesis of cyclic beta-(1,2)-glucans in strain TA-1 is controlled by end-product inhibition. These observations indicate that TA-1 cells become more permeable to cyclic glucans at high NaCl concentrations. The constant loss of glucans from cells grown in the presence of 200 mM NaCl prevented end-product inhibition and resulted in glucan accumulation of up to 1,600 mg/liter in the medium. |
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Authors:
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M W Breedveld; L P Zevenhuizen; A J Zehnder |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 174 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1992-11-13 Completed Date: 1992-11-13 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6336-42 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Agricultural University, Wageningen, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Chromatography Glucans / biosynthesis*, metabolism Magnesium / metabolism NAD / metabolism Osmotic Pressure Rhizobium leguminosarum / drug effects, growth & development, metabolism* Sodium Chloride / pharmacology Temperature Uridine Diphosphate Glucose / metabolism beta-Glucans* |
| Chemical | |
Reg. No./Substance:
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0/Glucans; 0/beta-Glucans; 133-89-1/Uridine Diphosphate Glucose; 53-84-9/NAD; 56-65-5/Adenosine Triphosphate; 7439-95-4/Magnesium; 7647-14-5/Sodium Chloride; 9051-99-4/beta-1,2-glucan |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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