Document Detail


Synthesis, chemical and enzymatic hydrolysis, and aqueous solubility of amino acid ester prodrugs of 3-carboranyl thymidine analogs for boron neutron capture therapy of brain tumors.
MedLine Citation:
PMID:  22889558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Various water-soluble L-valine-, L-glutamate-, and glycine ester prodrugs of two 3-Carboranyl Thymidine Analogs (3-CTAs), designated N5 and N5-2OH, were synthesized for Boron Neutron Capture Therapy (BNCT) of brain tumors since the water solubilities of the parental compounds proved to be insufficient in preclinical studies. The amino acid ester prodrugs were prepared and stored as hydrochloride salts. The water solubilities of these amino acid ester prodrugs, evaluated in phosphate buffered saline (PBS) at pH 5, pH 6 and pH 7.4, improved 48-6600 times compared with parental N5 and N5-2OH. The stability of the amino acid ester prodrugs was evaluated in PBS at pH 7.4, Bovine serum, and Bovine cerebrospinal fluid (CSF). The rate of the hydrolysis in all three incubation media depended primarily on the amino acid promoiety and, to a lesser extend, on the site of esterification at the deoxyribose portion of the 3-CTAs. In general, 3'-amino acid ester prodrugs were less sensitive to chemical and enzymatic hydrolysis than 5'-amino acid ester prodrugs and the stabilities of the latter decreased in the following order: 5'-valine > 5'-glutamate > 5'-glycine. The rate of the hydrolysis of the 5'-amino acid ester prodrugs in Bovine CSF was overall higher than in PBS and somewhat lower than in Bovine serum. Overall, 5'-glutamate ester prodrug of N5 and the 5'-glycine ester prodrugs of N5 and N5-2OH appeared to be the most promising candidates for preclinical BNCT studies.
Authors:
Sherifa Hasabelnaby; Ayman Goudah; Hitesh K Agarwal; Mosaad S M abd Alla; Werner Tjarks
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-27
Journal Detail:
Title:  European journal of medicinal chemistry     Volume:  55     ISSN:  1768-3254     ISO Abbreviation:  Eur J Med Chem     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-03     Completed Date:  2013-01-17     Revised Date:  2013-09-25    
Medline Journal Info:
Nlm Unique ID:  0420510     Medline TA:  Eur J Med Chem     Country:  France    
Other Details:
Languages:  eng     Pagination:  325-34     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Masson SAS. All rights reserved.
Affiliation:
Division of Pharmaceutical Organic Chemistry, College of Pharmacy, Helwan University, Ain Helwan, Cairo, Egypt. sherifajanaa@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry*
Animals
Boron Neutron Capture Therapy*
Brain Neoplasms / radiotherapy*
Cattle
Chemistry Techniques, Synthetic
Drug Design
Drug Stability
Esters
Hydrolysis
Prodrugs / chemical synthesis,  chemistry*,  metabolism
Solubility
Thymidine / analogs & derivatives*,  metabolism*,  therapeutic use
Water / chemistry*
Grant Support
ID/Acronym/Agency:
P30 CA016058/CA/NCI NIH HHS; R01 CA127935/CA/NCI NIH HHS; R01CA127935/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Esters; 0/Prodrugs; 50-89-5/Thymidine; 7732-18-5/Water
Comments/Corrections

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