Document Detail


Synthesis and characterization of poly(L-lactic acid-co-ethylene oxide-co-aspartic acid) and its interaction with cells.
MedLine Citation:
PMID:  16555113     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multiblock terpolymer of poly(L-lactic acid)/poly(ethylene oxide)/poly(L-aspartic acid), (PLLA/PEO/PAsp) was synthesized by ring opening polymerization of beta -benzyl L-aspartate N-carboxyanhydride, Asp(OBzl)-NCA with alpha-omega -hydroxy terminated triblock PLLA/PEO/PLLA copolymer. The resulting multiblock terpolymer was characterized by several techniques including Fourier transform infrared spectroscopy and differential scanning calorimetry.(1)H nuclear magnetic resonance spectra indicated the molar ratio of PLLA/PEO/PAsp (OBzl) to be 86/10/4. Thermal gravimetric analysis and environmental scanning electron microscopy data showed that PLLA/PEO/PAsp had crystalline and brittle structure. In order to improve its mechanical and physical properties, the terpolymer was blended with high molecular weight poly(L-lactic-co-glycolic acid) copolymer, PLGA(85/15) (M(w): 95000 gmol(-1)) in 25/75 and 50/50 mole ratios. The hydrolytical degradation properties of these polymers were studied. Degradation experiments were performed during a 48-day period in pH:7.4 phosphate-buffered saline (PBS) at 37 degrees C. The observed molecular weight losses were 91% and 67% for the 25/75 and 50/50 mixtures, respectively. In vitro attachment and growth of L929 mouse fibroblasts on these biopolymers were also investigated. Cell growth experiments indicated that the copolymer blend allowed the attachment and growth of cells.
Authors:
Okşan Karal-Yilmaz; Nilhan Kayaman-Apohan; Zülal Misirli; Kemal Baysal; Bahattin M Baysal
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of materials science. Materials in medicine     Volume:  17     ISSN:  0957-4530     ISO Abbreviation:  J Mater Sci Mater Med     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-23     Completed Date:  2006-08-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9013087     Medline TA:  J Mater Sci Mater Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  213-27     Citation Subset:  IM    
Affiliation:
TUBITAK, Research Institute for Genetic Engineering and Biotechnology, Gebze-Kocaeli, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartic Acid / chemistry*
Biocompatible Materials / chemical synthesis*
Biodegradation, Environmental / drug effects
Cell Line
Cell Survival / drug effects
Lactic Acid / chemical synthesis*,  pharmacology*
Materials Testing
Mice
Microscopy, Electron, Scanning / methods
Models, Molecular
Polyglycolic Acid / chemical synthesis*,  pharmacology*
Polymers / chemical synthesis*,  pharmacology
Surface Properties
Temperature
Tissue Engineering
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid; 56-84-8/Aspartic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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