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Synthesis and characterization of poly(3-hydroxyalkanoates) from Brassica carinata oil with high content of erucic acid and from very long chain fatty acids.
MedLine Citation:
PMID:  21035502     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Pseudomonas aeruginosa produced medium chain length poly(3-hydroxyalkanoates) (mcl-PHAs) when grown on substrates containing very long chain fatty acids (VLCFA, C>20). Looking for low cost carbon sources, we tested Brassica carinata oil (erucic acid content 35-48%) as an intact triglyceride containing VLCFA. Oleic (C(18:1)), erucic (C(22:1)), and nervonic (C(24:1)) acids were also employed for mcl-PHA production as model substrates. The polymers obtained were analyzed by GC of methanolyzed samples, GPC, (1)H and (13)C NMR, ESI MS of partially pyrolyzed samples, and DSC. The repeating units of such polymers were saturated and unsaturated, with a higher content of the latter in the case of the PHA obtained from B. carinata oil. Statistical analysis of the ion intensity in the ESI mass spectra showed that the PHAs from pure fatty acids are random copolymers, while the PHA from B. carinata oil is either a pure polymer or a mixture of polymers. Weight-average molecular weight varied from ca. 56,000g/mol for the PHA from B. carinata oil and oleic acid, to about 120,000g/mol for those from erucic and nervonic acids. The PHAs from erucic and nervonic acids were partially crystalline, with rubbery characteristics and a melting point (T(m)) of 50°C, while the PHAs from oleic acid and from B. carinata oil afforded totally amorphous materials, with glass transition temperatures (T(g)) of -52°C and -47°C, respectively.
Authors:
Giuseppe Impallomeni; Alberto Ballistreri; Giovanni Marco Carnemolla; Salvatore P P Guglielmino; Marco Sebastiano Nicolò; Maria Grazia Cambria
Publication Detail:
Type:  Journal Article     Date:  2010-10-28
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  48     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  137-45     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Istituto di Chimica e Tecnologia dei Polimeri, Consiglio Nazionale delle Ricerche, Via P. Gaifami, 18, 95126 Catania, Italy.
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