Document Detail

Synthesis and characterization of a novel extracellular polysaccharide by Rhodotorula glutinis.
MedLine Citation:
PMID:  11732715     Owner:  NLM     Status:  MEDLINE    
The aim of this work was to characterize an exopolysaccharide by Rhodotorula glutinis KCTC 7989 and to investigate the effect of the culture conditions on the production of this polymer. The extracellular polysaccharide (EPS) produced from this strain was a novel acidic heteropolysaccharide composed of neutral sugars (85%) and uronic acid (15%). The neutral sugar composition was identified by gas chromatography as mannose, fucose, glucose, and galactose in a 6.7:0.2:0.1:0.1 ratio. The molecular weight of purified EPS was estimated to be 1.0-3.8 x 10(5) Dalton, and the distribution of the molecular weight was very homogeneous (polydispersity index = 1.32). The EPS solution showed a characteristic of pseudoplastic non-Newtonian fluid at a concentration >2.0% in distilled water. The maximum EPS production was obtained when the strain was grown on glucose (30 g/L). Ammonium sulfate was the best suitable nitrogen source for EPS production. The highest yield of EPS was obtained at a carbon to nitrogen ratio of 15. The EPS synthesis was activated at the acidic range of pH 3.0-5.0 and increased when the pH of the culture broth decreased naturally to <2.0 during the fermentation. When the yeast was grown on glucose (30 g/L) and ammonium sulfate (2 g/L) at 22 degrees C at an initial pH of 4.0, EPS production was maximized (4.0 g/L), and the glucose-based production yield coefficient and carbon-based production yield coefficient were 0.30 g of EPS/g of glucose and 0.34 g (carbon of EPS)/g (carbon of glucose), respectively.
D H Cho; H J Chae; E Y Kim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  95     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-12-04     Completed Date:  2002-06-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  183-93     Citation Subset:  IM    
Department of Chemical Engineering, The University of Seoul, Korea.
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MeSH Terms
Glucose / metabolism
Hydrogen-Ion Concentration
Molecular Weight
Polysaccharides / chemistry*,  isolation & purification*,  metabolism
Rhodotorula / chemistry*,  growth & development
Spectroscopy, Fourier Transform Infrared
Reg. No./Substance:
0/Polysaccharides; 50-99-7/Glucose

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