Document Detail

Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides.
MedLine Citation:
PMID:  22840004     Owner:  NLM     Status:  In-Data-Review    
In this study we describe a method for highly specific enrichment of glycopeptides with boronic acid-functionalized chitosan polymeric nanospheres and matrix assisted laser desorption-ionization mass spectrometry (MALDI-MS). This is the first time chitosan has been used to create nanosphere support material for selective enrichment of glycopeptides by modification with glycidyl methacrylate (GMA) and derivatization with 3-aminophenylboronic acid (APB). Due to their multifunctional chemical moieties, these 20-100nm chitosan-GMA-APB nanospheres have unique properties, such as good dispersibility, good biocompatibility and chemical stability, as well as augmented specificity with glycopeptides. Enrichment conditions were optimized by using trypsin digested glycoprotein horseradish peroxidase. The high specificity of chitosan-GMA-APB nanospheres was demonstrated by effectively enriching glycopeptides from a digest mixture of horseradish peroxidase and nonglycoproteins (bovine serum albumin (BSA)).
Xiajuan Zou; Dan Liu; Lijun Zhong; Bin Yang; Yaxin Lou; Yuxin Yin
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Publication Detail:
Type:  Journal Article     Date:  2012-06-16
Journal Detail:
Title:  Carbohydrate polymers     Volume:  90     ISSN:  1879-1344     ISO Abbreviation:  Carbohydr Polym     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-07-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307156     Medline TA:  Carbohydr Polym     Country:  England    
Other Details:
Languages:  eng     Pagination:  799-804     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Proteomics Laboratory, Medical and Healthy Analytical Center, Peking University, Beijing 100191, China.
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