Document Detail


Synthesis, characterization, and in vitro evaluation of palmitoylated arabinogalactan with potential for liver targeting.
MedLine Citation:
PMID:  23298829     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Arabinogalactan (AG), a water soluble polysaccharide with more than 80mol% galactose units, was hydrophobized by covalent attachment of palmitoyl chains using a base-catalyzed esterification reaction with the objective of effective amalgamation of arabinogalactan in liposomes for targeting asialoglycoprotein receptors (ASGPR) on liver parenchymal cells. Palmitoylated AG (PAG) was characterized by physico-chemical parameters, IR, (1)H NMR, and (13)C NMR and molecular weight determination by gel permeation chromatography. PAG was incorporated in liposomes and the liposomes were characterized by dynamic light scattering, optical microscopy, zeta potential, and transmission electron microscopic (TEM) techniques. The liposomal system was evaluated for acute toxicity in swiss albino mice and was found to be safe. Targeting ability of PAG was confirmed by in vitro binding affinity to Ricinus communis agglutinin (RCA(120)), a lectin specific for galactose. The liposomal system with PAG was evaluated for cytotoxicity on HepG2, MCF7, and A549 cancer cell lines. Cytotoxicity study revealed enhanced activity on ASGPR-expressive HepG2 cells as compared to MCF7.
Authors:
Sanket M Shah; Pankaj O Pathak; Ankitkumar S Jain; Chandrashekhar R Barhate; Mangal S Nagarsenker
Related Documents :
23138839 - Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) bas...
7328639 - Scanning immuno-electron microscopy of human leukaemia and lymphoma cells: a comparativ...
23438859 - Painting by numbers: increasing the parts list for chromatin domains.
23035079 - Opioids disrupt pro-nociceptive modulation mediated by raphe magnus.
23017599 - Formaldehyde induces rapid glutathione export from viable oligodendroglial oln-93 cells.
24523759 - Growth inhibition and apoptosis induction of salvia chloroleuca on mcf-7 breast cancer ...
2824529 - Transfection of neonatal rat schwann cells with sv-40 large t antigen gene under contro...
23138839 - Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) bas...
7956829 - Genetic control of sex determination in the germ line and soma of the housefly, musca d...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-8
Journal Detail:
Title:  Carbohydrate research     Volume:  367C     ISSN:  1873-426X     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  41-47     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Pharmaceutics, Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai 400098, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The analysis of the outcomes and factors related to iliac-obturator involvement in cutaneous melanom...
Next Document:  Neurosensory assessments of migraine.