| Synthesis, characterization and hybridization studies of an alternate nucleo-epsilon/gamma-peptide: complexes formation with natural nucleic acids. | |
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MedLine Citation:
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PMID: 19067107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to develop new oligodeoxyribonucleotide (ODN) analogs to be used in biotechnological applications, we report here the synthesis, characterization and nucleic acid binding studies of novel nucleopeptides, that we called epsilon-lys/gamma-dabPNAs, containing a backbone of alternated L-diaminobutyric acid and L-lysine moieties. Exploring the hybridization properties of the new ODN analog, we found, by circular dichroism and UV spectroscopies, that a homothymine epsilon-lys/gamma-dabPNA hexamer binds both DNA and RNA of complementary sequence. Furthermore, human serum stability assays on the alternate nucleopeptide evidenced a noteworthy degradation resistance. These results encourage us to deepen the knowledge of this analog, in order to evaluate its possible use in antigene/antisense or diagnostic applications. |
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Authors:
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G N Roviello; D Musumeci; C Pedone; E M Bucci |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-07 |
Journal Detail:
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Title: Amino acids Volume: 38 ISSN: 1438-2199 ISO Abbreviation: Amino Acids Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-26 Completed Date: 2010-04-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: Austria |
Other Details:
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Languages: eng Pagination: 103-11 Citation Subset: IM |
Affiliation:
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Istituto di Biostrutture e Bioimmagini-CNR, Via Mezzocannone 16, 80134, Napoli, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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chemistry Humans Molecular Structure Nucleic Acid Hybridization Oligodeoxyribonucleotides / chemical synthesis, chemistry* Peptide Nucleic Acids / chemistry* Peptides / chemical synthesis, chemistry* RNA / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Oligodeoxyribonucleotides; 0/Peptide Nucleic Acids; 0/Peptides; 63231-63-0/RNA; 9007-49-2/DNA |
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