| Synthesis and characterization of a highly fluorescent peptidyl-phosphatidylethanolamine. | |
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MedLine Citation:
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PMID: 4031655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The synthesis of a fluorescent lipid for use in studies of immune recognition of model membranes is described. The molecule has the basic structure HAPTEN-SPACER-LIPID, where fluorescein is the hapten, an oligopeptide (triglycine) is the spacer, and dipalmitoylphosphatidylethanolamine (DPPE) is the lipid. The spacer, which is necessary for immunological reactivity, is first linked via a peptide bond to DPPE. The free N-terminus of the peptidyl-DPPE is then reacted with 5-dichlorotriazinylaminofluorescein (DCTAF) to yield fluoresceinchlorotriazinyltriglycyl-DPPE (FG3P). The structure is confirmed by mass spectrometry and Fourier transform NMR. When FG3P is incorporated into phospholipid vesicles it retains the brilliant fluorescence and high-affinity immunological reactivity of fluorescein. The general synthesis scheme may prove useful in other membrane and lipoprotein applications. |
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Authors:
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A Petrossian; A B Kantor; J C Owicki |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of lipid research Volume: 26 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1985 Jun |
Date Detail:
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Created Date: 1985-10-03 Completed Date: 1985-10-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 767-73 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescein Fluoresceins Fluorescent Dyes* Glycine Mass Spectrometry Oligopeptides Phosphatidylethanolamines / chemical synthesis* |
| Grant Support | |
ID/Acronym/Agency:
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2-S07-RR-7006/RR/NCRR NIH HHS; R01-AI-19605-02/AI/NIAID NIH HHS; RR01614/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Fluorescent Dyes; 0/Oligopeptides; 0/Phosphatidylethanolamines; 2321-07-5/Fluorescein; 3026-45-7/1,2-dipalmitoyl-3-phosphatidylethanolamine; 56-40-6/Glycine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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