Document Detail


Synthesis and characterization of elastic and macroporous chitosan-gelatin cryogels for tissue engineering.
MedLine Citation:
PMID:  18701361     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Elastic chitosan-gelatin cryogels of varying concentration of polymer precursors have been synthesized using glutaraldehyde as a crosslinking agent. The optimum co-polymer ratio of chitosan to gelatin was found to be 1:4 at the temperature of -12 degrees C for synthesis of chitosan-gelatin hybrid cryogels. chitosan-gelatin cryogels synthesized with low viscosity chitosan were morphologically better than those formed with medium and high viscosity chitosan. Pore diameters of chitosan-gelatin cryogels as measured by scanning electron microscopy (SEM) was in the range of 30-100microm. While mercury porosimetry analysis revealed the majority of pores of the scaffold lying in the range of 30-50microm. Porosity of chitosan-gelatin cryogels was found to be greater than 90% using Archimedes's principle. Unconfined compression tests showed significant elasticity of chitosan-gelatin cryogels and maintained their physical integrity even after compressing them up to 80% of their original length. The elastic modulus varied in the range of 36-39kPa. Cyclic deformation analysis performed by compression of chitosan-gelatin cryogels with varying strains (10, 20 and 40%) showed no cracking or any significant deformation. The degradation of chitosan-gelatin cryogels was found up to 13.58+/-1.52% at 37 degrees C within 8 weeks of incubation under sterile conditions and the cryogels swelled up to 90% of their capacity within two min. Efficient cell adherence, proliferation and extracellular matrix (ECM) secretion was observed by growing fibroblast (Cos-7) cell line on chitosan-gelatin hybrid cryogels which indicate potential of the material for tissue engineering applications.
Authors:
Neeraj Kathuria; Anuj Tripathi; Kamal K Kar; Ashok Kumar
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-25
Journal Detail:
Title:  Acta biomaterialia     Volume:  5     ISSN:  1878-7568     ISO Abbreviation:  -     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-11-26     Completed Date:  2009-03-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101233144     Medline TA:  Acta Biomater     Country:  England    
Other Details:
Languages:  eng     Pagination:  406-18     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur 208016, Uttar Pradesh, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry
Blood Proteins / chemistry*
COS Cells
Cell Adhesion
Cercopithecus aethiops
Chitosan / chemistry*
Elasticity
Fibronectins / chemistry*
Gelatin / chemistry*
Hydrogels
Microscopy, Electron, Scanning
Porosity
Pressure
Temperature
Time Factors
Tissue Engineering / methods*
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Blood Proteins; 0/Fibronectins; 0/Hydrogels; 0/cryogel; 9000-70-8/Gelatin; 9012-76-4/Chitosan

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