Document Detail


Synthesis and characterization of 2-mercaptoethanesulfonic acid albumin.
MedLine Citation:
PMID:  9689429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Autoimmune patients treated with ifosfamide (CAS 3778-73-2) and mesna (2-mercaptoethanesulfonic acid, CAS 3375-50-6) in some cases suffered from severe allergic reactions that were proposed to be due to mesna linked to serum albumin by a disulfide bond. To prove the existence of the hypothetic mesna albumin adduct in vivo it was synthesized: The free thiol group of albumin (molecular mass determined by MALDI spectroscopy: 67009 Da) was converted to S-phenylsulfonyl albumin and reacted with mesna to albumin mesna (molecular mass: 67159 Da). In an alternative synthesis albumin was incubated with mesna at pH 8, 40 degrees C (molecular mass of the adduct: 67166 Da).
Authors:
H H Bauer; S Ehmig; J W Engels; G Voelcker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arzneimittel-Forschung     Volume:  48     ISSN:  0004-4172     ISO Abbreviation:  Arzneimittelforschung     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-09-17     Completed Date:  1998-09-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372660     Medline TA:  Arzneimittelforschung     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  691-3     Citation Subset:  IM    
Affiliation:
Universitätsklinikum, Zentrum der Biologischen Chemie, Institut für Biochemie II, Johann Wolfgang Goethe-Universität, Frankfurt/Main, Germany.
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MeSH Terms
Descriptor/Qualifier:
Albumins / chemistry*
Chromatography, Affinity
Humans
Hydrogen-Ion Concentration
Mesna / analogs & derivatives*,  chemical synthesis,  chemistry*
Molecular Weight
Serum Albumin / chemical synthesis,  chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical
Reg. No./Substance:
0/2-mercaptoethanesulfonic acid albumin; 0/Albumins; 0/Serum Albumin; 19767-45-4/Mesna

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