Document Detail


Synthesis and characterisation of a new pH-sensitive amphotericin B--poly(ethylene glycol)-b-poly(L-lysine) conjugate.
MedLine Citation:
PMID:  17336066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper reports on the synthesis, characterisation, and efficiency of a new intravenous conjugate of amphotericin B (AMB). Twelve molecules of AMB were attached to block copolymer poly(ethylene glycol)-b-poly(L-lysine) via pH-sensitive imine linkages. In vitro drug release studies demonstrated the conjugate (M(w)=26,700) to be relatively stable in human plasma and in phosphate buffer (pH 7.4, 37 degrees C). Controlled release of AMB was observed in acidic phosphate buffer (pH 5.5, 37 degrees C) with the half-life of 2 min. The LD(50) value determined in vivo (mouse) is 45 mg/kg.
Authors:
Milos Sedlák; Martin Pravda; Lenka Kubicová; Petra Mikulcíková; Karel Ventura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-07
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  17     ISSN:  0960-894X     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-06     Completed Date:  2007-07-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  2554-7     Citation Subset:  IM    
Affiliation:
Department of Organic Chemistry, University of Pardubice, Pardubice, Czech Republic. milos.sedlak@upce.cz
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MeSH Terms
Descriptor/Qualifier:
Amphotericin B / analogs & derivatives*,  chemical synthesis*
Antifungal Agents / chemical synthesis*,  pharmacology
Chemistry, Pharmaceutical / methods*
Drug Design
Hydrogen-Ion Concentration
Models, Chemical
Molecular Conformation
Polyethylene Glycols / chemistry*
Polylysine / chemistry*
Polymers / chemistry
Spectrophotometry, Ultraviolet / methods
Temperature
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Polyethylene Glycols; 0/Polymers; 1397-89-3/Amphotericin B; 25104-18-1/Polylysine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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