Document Detail

Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles.
MedLine Citation:
PMID:  22609600     Owner:  NLM     Status:  Publisher    
We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α(v)β(3)) and MCF-7 cells (human breast cancer cells, express low level of integrin α(v)β(3)) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α(v)β(3)-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.
Myung-Ik Yoo; Yeong-Ju Seo; Kyu-Sil Choi; Jeong Sook Ha; Kyoungja Woo
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-13
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  97C     ISSN:  1873-4367     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-5-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  -    
Other Details:
Languages:  ENG     Pagination:  175-181     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Molecular Recognition Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul 130-650, Republic of Korea; Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Republic of Korea.
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