| Synthesis of cell-adhesive dextran hydrogels and macroporous scaffolds. | |
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MedLine Citation:
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PMID: 16793132 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dextran hydrogels have been previously investigated as drug delivery vehicles and more recently as macroporous scaffolds; however, the non-cell-adhesive nature of dextran has limited its utility for tissue engineering. To overcome this limitation, macroporous scaffolds of methacrylated dextran (Dex-MA) copolymerized with aminoethyl methacrylate (AEMA) were synthesized, thereby introducing primary amine groups for covalent immobilization of extracellular-matrix-derived peptides. The amino group density for hydrogels copolymerized with 0.5 wt% AEMA was found to be 36.1+/-0.4 micromol/cm(3) by elemental analysis. To further enhance cellular interaction, poly(Dex-MA-co-AEMA) hydrogels were modified with either CRGDS or a mixture of CDPGYIGSR and CQAASIKVAV (1:1, v/v) using sulfo-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC). The immobilized peptide concentration was determined using amino acid analysis at: 2.6+/-0.9 micromol/cm(3) for CRGDS-derived hydrogels and 2.2+/-0.3 micromol/cm(3) plus 1.9+/-0.2 micromol/cm(3) for CDPGYIGSR plus CQAASIKVAV-derived hydrogels, respectively. Cellular interactions of primary embryonic chick dorsal root ganglia (DRGs) were compared on the hydrogels. Cell adhesion and neurite outgrowth on poly(Dex-MA) increased with copolymerization of AEMA and further improved with peptide modification and significantly for CDPGYIGSR/CQAASIKVAV-derived poly(Dex-MA-co-AEMA) hydrogels. Moreover, DRGs penetrated within the first 600 microm of the scaffolds, thereby demonstrating the potential of this scaffold for guided cell and axonal regeneration in vivo. |
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Authors:
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Stéphane G Lévesque; Molly S Shoichet |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-21 |
Journal Detail:
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Title: Biomaterials Volume: 27 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-07-25 Completed Date: 2006-11-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 5277-85 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ont., Canada M5S 3E5. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adhesives
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chemical synthesis,
chemistry*,
pharmacology Animals Cell Adhesion / drug effects Cells, Cultured Chick Embryo Dextrans / chemistry* Ethylamines / chemistry* Ganglia, Spinal / cytology, drug effects Hydrogels / chemical synthesis, chemistry*, pharmacology Methacrylates / chemistry* Neurites / drug effects Oligopeptides / chemistry Porosity Tissue Adhesions Tissue Engineering* |
| Chemical | |
Reg. No./Substance:
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0/Adhesives; 0/Ethylamines; 0/Hydrogels; 0/Methacrylates; 0/Oligopeptides; 0/aminoethyl methacrylate; 0/methacrylated dextran; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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