Document Detail

Synthesis of caffeic acid and p-hydroxybenzoic acid molecularly imprinted polymers and their application for the selective extraction of polyphenols from olive mill waste waters.
MedLine Citation:
PMID:  18221745     Owner:  NLM     Status:  MEDLINE    
Using caffeic acid and p-hydroxybenzoic acid as templates, two molecularly imprinted polymers (MIPs) were prepared that were used for isolation of polyphenols from olive mill waste water samples (OMWWs) without previous pre-treatment. For the preparation of the caffeic acid MIPs 4-vinylpyridine, allylurea, allylaniline and methacrylic acid were tested as functional monomers, ethylene glycol dimethylacrylate (EDMA), pentaerythritol trimethylacrylate (PETRA) and divinylbenzene 80 (DVB80) as cross-linkers and tetrahydrofuran as porogen. For p-hydroxybenzoic acid 4-vinylpyridine, allylurea and allylaniline were tested as functional monomers, EDMA and PETRA as cross-linkers and acetonitrile as porogen. The performance of the synthesized polymers was evaluated against seven structurally related compounds by means of polymer-based HPLC. The two polymers that presented the most interesting properties were further evaluated by batch rebinding and from the derived isotherms their capacity and binding strength were determined. Using solid-phase extraction (SPE), their ability to recognize and bind the template molecule from an aqueous solution as well as the pH dependence of the binding strength were explored. After establishing the best SPE protocol, an aqueous model mixture of compounds and a raw OMWWs sample were loaded on the two best polymers. The result of the consecutive use of the two polymers on the same sample was explored. It was concluded that acidic conditions favour the recognition abilities of both polymers and that they can be used for a quick and efficient isolation of the polyphenol fraction directly from raw OMWW.
Chrysa Michailof; Panagiotis Manesiotis; Costas Panayiotou
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Publication Detail:
Type:  Journal Article     Date:  2008-01-06
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1182     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-04     Completed Date:  2008-05-19     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  25-33     Citation Subset:  IM    
Department of Chemical Engineering, Laboratory of Physical Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
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MeSH Terms
Caffeic Acids / chemistry*
Chromatography, High Pressure Liquid
Flavonoids / chemistry*,  isolation & purification
Hydrogen-Ion Concentration
Hydroxybenzoic Acids / chemistry*
Industrial Waste*
Molecular Imprinting
Molecular Structure
Olea / chemistry*
Phenols / chemistry*,  isolation & purification
Polymers / chemical synthesis,  chemistry*
Reproducibility of Results
Solid Phase Extraction / methods
Reg. No./Substance:
0/Caffeic Acids; 0/Flavonoids; 0/Hydroxybenzoic Acids; 0/Industrial Waste; 0/Phenols; 0/Polymers; 0/polyphenols; 331-39-5/caffeic acid

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