| Synthesis and biophysical studies on fluorescently labeled oligodeoxyribonucleotides. | |
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MedLine Citation:
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PMID: 17578747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two highly fluorescent compounds, viz. 6-(6-isobutyrylamino-1,3-dioxo-1 H,3H-benzo[de]isoquinolin-2-yl)-hexanoic acid and 6-(6-dimethylamino-1,3-dioxo-1 H,3H-benzo[de]isoqu-inolin-2-yl)-hexanoic acid have been synthesized, characterized, and attached to 12-mer oligodeoxyribonucleotides at their 5'-end using suitable linker molecule. These labeled oligodeoxyribonucleotides have shown appreciable fluorescence even at 0.0019 microM concentrations. Thermal denaturation studies have shown comparatively higher Tm values when oligodeoxyribonucleotides are labeled. These labeled oligodeoxyribonucleotides have been purified on RP-HPLC utilizing their hydrophobicity and on polyacrylamide gel because of their easy detection due to fluorescence. |
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Authors:
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Shipra Singh; Pradeep Kumar; K C Gupta; Ramendra K Singh |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleosides, nucleotides & nucleic acids Volume: 26 ISSN: 1525-7770 ISO Abbreviation: Nucleosides Nucleotides Nucleic Acids Publication Date: 2007 |
Date Detail:
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Created Date: 2007-06-20 Completed Date: 2007-10-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892832 Medline TA: Nucleosides Nucleotides Nucleic Acids Country: United States |
Other Details:
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Languages: eng Pagination: 521-31 Citation Subset: IM |
Affiliation:
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Nucleic Acids Research Laboratory, Department of Chemistry, University of Allahabad, Allahabad, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Fluorescence* Hexanoic Acids / chemical synthesis, chemistry Isoquinolines / chemical synthesis, chemistry Oligodeoxyribonucleotides / chemical synthesis*, chemistry* Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Transition Temperature |
| Chemical | |
Reg. No./Substance:
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0/6-(6-dimethylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; 0/6-(6-isobutyrylamino-1,3-dioxo-1H,3H-benzo(de)isoquinolin-2-yl)hexanoic acid; 0/Hexanoic Acids; 0/Isoquinolines; 0/Oligodeoxyribonucleotides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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