| Synthesis of biologically active interleukin 2 by Xenopus oocytes in response to poly(A)-RNA from a gibbon T-cell line. | |
| | |
MedLine Citation:
|
PMID: 6173379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The poly(A)-containing RNA isolated from a gibbon lymphosarcoma cell line, MLA144, known to release Interleukin 2 (IL2) was microinjected into Xenopus oocytes. The incubation medium from injected oocytes stimulated the DNA synthesis of an IL2-dependent cell culture and also maintained its viability. DNA-stimulating activity appeared in the oocyte incubation medium 6 h after injection and continued to accumulate for at least 96 h. Thymidine incorporation by the IL2-dependent cells is proportional to the concentration of oocyte incubation medium added and the IL2 activity produced by the oocytes is proportional to the amount of poly(A)-RNA injected. Incubation medium of oocytes injected with RNA from another T-cell line, 6G1, which does not produce IL2, did not contain DNA-stimulating activity. The same assay also showed that the IL2 mRNA sedimented at 14-16 S in a nondenaturing sucrose gradient. A series of monoclonal antibodies prepared against human IL2 neutralized the DNA-stimulating activity released by the injected oocytes to the same extent as they neutralized human IL2. |
| | |
Authors:
|
Y Lin; B M Stadler; H Rabin |
Related Documents
:
|
11873829 - Fate of plasmid dna encoding infectious bursal disease virus vp2 capsid protein gene af... 23146099 - Mutations arising during repair of chromosome breaks. 23566839 - Dna-damage response in chromatin of ribosomal genes and the surrounding genome. 18507679 - Enhanced resistance against systemic candida albicans infection in mice treated with c.... 23029299 - Associations between the expression of epigenetically regulated genes and the expressio... 12526829 - Gene expression and mucosal immune responses after vaginal dna immunization in mice usi... 17349959 - Mdm2-regulated degradation of hipk2 prevents p53ser46 phosphorylation and dna damage-in... 22261329 - Dna damage-induced apoptosis: from specific dna lesions to the dna damage response and ... 21727979 - A microfluidic system for fast detection of mitochondrial dna deletion. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 257 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1982 Feb |
Date Detail:
|
Created Date: 1982-04-12 Completed Date: 1982-04-12 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1587-90 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Monoclonal Callitrichinae Cell Line DNA Replication Female Hybridomas / immunology Hylobates Interleukin-2 / genetics* Kinetics Lymphokines / genetics* Oocytes / metabolism* Ovum / metabolism* Poly A / genetics* RNA / genetics* RNA, Messenger T-Lymphocytes Xenopus |
| Grant Support | |
ID/Acronym/Agency:
|
N01-C0-75380//PHS HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Interleukin-2; 0/Lymphokines; 0/RNA, Messenger; 24937-83-5/Poly A; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Selective labeling and rotational diffusion of the ADP/ATP translocator in the inner mitochondrial m...
Next Document: Presence of 2',5'-oligo(A) and of enzymes that synthesize, bind, and degrade 2',5'-oligo(A) in HeLa ...