Document Detail

Synthesis of biologically active interleukin 2 by Xenopus oocytes in response to poly(A)-RNA from a gibbon T-cell line.
MedLine Citation:
PMID:  6173379     Owner:  NLM     Status:  MEDLINE    
The poly(A)-containing RNA isolated from a gibbon lymphosarcoma cell line, MLA144, known to release Interleukin 2 (IL2) was microinjected into Xenopus oocytes. The incubation medium from injected oocytes stimulated the DNA synthesis of an IL2-dependent cell culture and also maintained its viability. DNA-stimulating activity appeared in the oocyte incubation medium 6 h after injection and continued to accumulate for at least 96 h. Thymidine incorporation by the IL2-dependent cells is proportional to the concentration of oocyte incubation medium added and the IL2 activity produced by the oocytes is proportional to the amount of poly(A)-RNA injected. Incubation medium of oocytes injected with RNA from another T-cell line, 6G1, which does not produce IL2, did not contain DNA-stimulating activity. The same assay also showed that the IL2 mRNA sedimented at 14-16 S in a nondenaturing sucrose gradient. A series of monoclonal antibodies prepared against human IL2 neutralized the DNA-stimulating activity released by the injected oocytes to the same extent as they neutralized human IL2.
Y Lin; B M Stadler; H Rabin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  257     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-04-12     Completed Date:  1982-04-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1587-90     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal
Cell Line
DNA Replication
Hybridomas / immunology
Interleukin-2 / genetics*
Lymphokines / genetics*
Oocytes / metabolism*
Ovum / metabolism*
Poly A / genetics*
RNA / genetics*
RNA, Messenger
Grant Support
N01-C0-75380//PHS HHS
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Interleukin-2; 0/Lymphokines; 0/RNA, Messenger; 24937-83-5/Poly A; 63231-63-0/RNA

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