| Synthesis and biological activity of new iodoacetamide derivatives on mutants of squalene-hopene cyclase. | |
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MedLine Citation:
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PMID: 16196424 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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New iodoacetamide derivatives, containing a dodecyl or a squalenyl moiety, were synthesized. The effect of these new thiol-reacting molecules was studied on two mutants of Alicyclobacillus acidocaldarius squalene-hopene cyclase constructed especially for this purpose. In the quintuple mutant, all five cysteine residues of the enzyme are substituted with serine; in the sextuple mutant, this quintuple substitution is accompanied by the substitution of aspartate D376, located at the enzyme's active site, with a cysteine. N-Dodecyliodoacetamide had little activity toward either mutant, whereas N-squalenyliodoacetamide showed a stronger effect on the sextuple than on the quintuple mutant, as expected. |
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Authors:
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Maurizio Ceruti; Gianni Balliano; Flavio Rocco; Alexander Lenhart; Georg E Schulz; Francesco Castelli; Paola Milla |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 40 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-10-03 Completed Date: 2006-01-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: United States |
Other Details:
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Languages: eng Pagination: 729-35 Citation Subset: IM |
Affiliation:
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Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, 10125 Torino, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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enzymology Intramolecular Transferases / genetics, metabolism* Iodoacetamide / chemical synthesis*, pharmacology* Magnetic Resonance Spectroscopy Mutation* Spectrometry, Mass, Electrospray Ionization |
| Chemical | |
Reg. No./Substance:
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144-48-9/Iodoacetamide; EC 5.4.-/Intramolecular Transferases; EC 5.4.99.-/squalene-hopene cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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