| Synthesis and bioassay of a boron-dipyrromethene derivative of estradiol for fluorescence imaging in vivo. | |
| | |
MedLine Citation:
|
PMID: 22542503 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
C7α-substituted estradiols bind to estrogen receptors in cell nuclei, yet these derivatives remain little used in bioimaging. Here, we describe a fluorescent derivative of estradiol (E2) with a boron-dipyrromethene (BODIPY) moiety attached to C7α, synthesized by olefin metathesis reaction of 7α-allylestradiol and 9-decenyl-BODIPY. In ovariectomized rats and non-ovariectomized mice, E2-BODIPY promoted the growth of uterine tissue similar to the effect of estradiol. Twenty-four hours after subcutaneous injection of E2-BODIPY in non-ovariectomized mice, we observed fluorescence of E2-BODIPY in the nuclei of uterine epithelial cells. Our findings suggest that fluorescence microscopy can localize this derivative in E2-responsive cells during normal development and tumorigenesis in vivo. |
| | |
Authors:
|
Mayumi Okamoto; Shun Kobayashi; Hiroshi Ikeuchi; Shunji Yamada; Korehito Yamanouchi; Kazumichi Nagasawa; Shun Maekawa; Takashi Kato; Isao Shimizu |
Related Documents
:
|
22593653 - Prevalence of goblet cell metaplasia in endocervical and endometrial adenocarcinoma : a... 9636183 - Fission yeast orb6, a ser/thr protein kinase related to mammalian rho kinase and myoton... 12020433 - Exposure to 2.45 ghz electromagnetic fields induces hsp70 at a high sar of more than 20... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-4-19 |
Journal Detail:
|
Title: Steroids Volume: - ISSN: 1878-5867 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
|
Created Date: 2012-4-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0404536 Medline TA: Steroids Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
|
Department of Applied Chemistry, School of Advanced Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan; Research Institute for Science and Engineering, School of Advanced Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Synthesis of 2-[(11)C]methoxy-3,17?-O,O-bis(sulfamoyl)estradiol as a new potential PET agent for ima...
Next Document: Estrogen receptor beta dependent attenuation of cytokine-induced cyclooxygenase-2 by androgens in hu...