Document Detail

Synthesis and assessment of the relative toxicity of the oxidised derivatives of campesterol and dihydrobrassicasterol in U937 and HepG2 cells.
MedLine Citation:
PMID:  22561884     Owner:  NLM     Status:  Publisher    
The cytotoxic effects of the oxidised derivatives of the phytosterols, stigmasterol and β-sitosterol, have previously been shown to be similar but less potent than those of the equivalent cholesterol oxides in the U937 cell line. The objective of the present study was to compare the cytotoxic effects of the oxidised derivatives of synthetic mixtures of campesterol and dihydrobrassicasterol in both the U937 and HepG2 cell lines. The parent compounds consisted of a campesterol: dihydrobrassicasterol mix at a ratio of 2:1 (2CMP:1DHB) and a dihydrobrassicasterol:campesterol mix at a ratio of 3:1 (3DHB:1CMP). The 2CMP:1DBH oxides were more cytotoxic in the U937 cells than the 3DBH:1CMP oxides but the difference in cytotoxicity was less marked in the HepG2 cells. The order of toxicity of the individual oxidation products was found to be similar to that previously observed for cholesterol, β-sitosterol and stigmasterol oxidation products in the U937 cell line. There was an increase in apoptotic nuclei in U937 cells incubated with the 7-keto and 7β-OH derivatives of both 2CMP:1DHB and 3DHB:1CMP and also in the presence of 3DHB:1CMP-3β,5α,6β-triol and 2CMP:1DHB-5β,6β-epoxide. An additional oxidation product synthesised from 2CMP:1DHB, 5,6,22,23-diepoxycampestane, was cytotoxic but did not induce apoptosis. These results signify the importance of campesterol oxides in the overall paradigm of phytosterol oxide cytotoxicity.
Yvonne O'Callaghan; Olivia Kenny; Niamh M O'Connell; Anita R Maguire; Florence O McCarthy; Nora M O'Brien
Related Documents :
22890174 - Diterpenoids from the buds of pinus banksiana lamb.
18156774 - Molecular modeling, synthesis, and screening of new bacterial quorumsensing antagonists.
1967314 - 4-(1,2,5,6-tetrahydro-1-alkyl-3-pyridinyl)-2-thiazolamines: a novel class of compounds ...
9871534 - New nonsteroidal androgen receptor modulators based on 4-(trifluoromethyl)-2(1h)-pyrrol...
25317954 - Mesoporous carbon-vanadium oxide films by resol-assisted, triblock copolymer-templated ...
23789904 - Studies on nonenzymatic oxidation mechanisms in neobetanin, betanin, and decarboxylated...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-26
Journal Detail:
Title:  Biochimie     Volume:  -     ISSN:  1638-6183     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-5-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Masson SAS.
School of Food and Nutritional Sciences, University College Cork, Cork, Ireland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Next Document:  Multiple semantic processes at different levels of syntactic hierarchy: Does the higher-level proces...