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Synthesis of artificial antigens of amoxicillin and preparation of monoclonal antibody to amoxicillin.
MedLine Citation:
PMID:  21722531     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM: To synthesize two kinds of amoxicillin artificial antigen and to establish hybridoma cell lines secreting monoclonal antibody against amoxillin. METHODS: Amoxicillin were respectively coupled with ovalbumin(OVA) and bovine serum albumin(BSA) by using N-hydroxysuc-cinimide active ester (NHS) method to prepared the immunogen and detection antigen. Molar ratio of conjugates were detected by ultraviolet spectrum analysis. BALB/c mice was immunized with AMX-OVA. The hybridomas were obtained by fusing mouse myeloma cells Sp2/0 with splenocytes from the mice immunized with AMX-OVA. The aseites with mAb were purified by octanoic acid-saturated ammonium sulphate method. Isotype was determinated by Mouse Isotype kit (Bio-RadLabs.).RESULTS: UV spectra shows overlay features between a small molecular compound and carrier protein. Five hybridoma cell strains secreting specific mAb against amoxicillin were obtained and the five Amb belong to IgG1 kappa isotype. The limit of detection of AMX was 0.25 ng/mL and but there is mild degree of cross-reactions with ampicillin with same structure of β-lactam ring. CONCLUSION: The anti-AMX Amb could be used to develop immunoassay for detection of residues.
Authors:
Tian Xue; Kai Song; Xiao-Ping Xue
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology     Volume:  27     ISSN:  1007-8738     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139110     Medline TA:  Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi     Country:  -    
Other Details:
Languages:  CHI     Pagination:  770-772     Citation Subset:  -    
Affiliation:
Faculty of Life Science, Northwestern Ploytechnical University, Xi'an 710072, China.
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