Document Detail

Synthesis of alpha-fetoprotein by rat ascites hepatoma cells.
MedLine Citation:
PMID:  54030     Owner:  NLM     Status:  MEDLINE    
AH-66 rat ascites hepatoma cells incorporated [14C]leucine into the AFP fraction. In a cell-free system, hepatoma ribosomes were found to be active in AFP synthesis whereas the supernatant fraction from hepatoma had no specific effect on AFP production. The amount of [14C]leucine incorporated in AFP by membrane-bound polysomes was 20 to 90 times higher than that by free polysomes, suggesting that AFP is mainly synthesized on membrane-bound polysomes. DBcAMP inhibited the growth of hepatoma cells. However, the incorporation of [14C]leucine into the AFP fraction as well as into total proteins was stimulated by DBcAMP.
K Kanai; Y Endo; T Oda; Y Kaneko
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  259     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  1975 Aug 
Date Detail:
Created Date:  1976-02-09     Completed Date:  1976-02-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  29-36     Citation Subset:  IM    
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MeSH Terms
Bucladesine / pharmacology
Carcinoma, Hepatocellular / metabolism*
Fetal Proteins / biosynthesis*
Leucine / metabolism
Liver / metabolism
Liver Neoplasms
Neoplasms, Experimental / metabolism
Polyribosomes / metabolism
Protein Biosynthesis / drug effects
Ribosomes / metabolism
Theophylline / pharmacology
alpha-Fetoproteins / biosynthesis*
Reg. No./Substance:
0/Fetal Proteins; 0/alpha-Fetoproteins; 362-74-3/Bucladesine; 58-55-9/Theophylline; 61-90-5/Leucine

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