Document Detail


Synthesis of acetylcholine by excitatory motoneurons in central nervous system of the leech.
MedLine Citation:
PMID:  15049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. A study was made of the synthesis of acetylcholine (ACh) and other transmitters by the cell bodies of functionally identified neurons in leech segmental ganglia. 2. Choline acetyltransferase, the synthetic enzyme for ACh, was detected in excitatory motoneurons but not in mechanosensory cells or Retzius cells. The ability of motoneurons to synthesize ACh was also demonstrated by their accumulation of [3H]ACh following incubation of segmental ganglia with [3H]choline. [3H]ACh was not detected in the other cell types. When eserine was included in [3H]choline incubations, the amount of [3H]ACh in motoneurons increased severalfold and small amounts of [3H]ACh (1% that in motor cells) appeared in extracts of sensory and Retzius cells. 3. In addition to [3H]ACh segmental ganglia synthesized [3H]5-HT, [3H]gamma-aminobutyric acid, [3H]dopamine, and [3H]octopamine from exogenous, labeled precursors. None of these labeled transmitters was detected in identified neurons except [3H]5-HT, which was found in Retzius cells. 4. These results provide biochemical evidence that excitatory motoneurons in the leech are cholinergic, but leave open the identity of the sensory transmitter(s).
Authors:
P B Sargent
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neurophysiology     Volume:  40     ISSN:  0022-3077     ISO Abbreviation:  J. Neurophysiol.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-05-20     Completed Date:  1977-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375404     Medline TA:  J Neurophysiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  453-60     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / biosynthesis*
Animals
Choline / metabolism
Choline O-Acetyltransferase / metabolism
Dopamine / biosynthesis
Ganglia / cytology
Leeches
Motor Neurons / drug effects,  enzymology,  metabolism*
Neurotransmitter Agents / biosynthesis*
Octopamine / biosynthesis
Physostigmine / pharmacology
Serotonin / biosynthesis
gamma-Aminobutyric Acid / biosynthesis
Chemical
Reg. No./Substance:
0/Neurotransmitter Agents; 104-14-3/Octopamine; 50-67-9/Serotonin; 51-84-3/Acetylcholine; 56-12-2/gamma-Aminobutyric Acid; 57-47-6/Physostigmine; 62-49-7/Choline; EC 2.3.1.6/Choline O-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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