| Synthesis of NAD+ in erythrocytes incubated with nicotinic acid and the effect of di-(2-ethyl hexyl) phthalate (DEHP). | |
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MedLine Citation:
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PMID: 9854904 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Synthesis of NAD+ from nicotinic acid by erythrocytes incubated in SAGM phosphate solution and effect of di-[2-ethyl hexyl] phthalate, a plasticizer commonly used in PVC blood/component storage bags, on this synthesis was studied. Erythrocytes are able to synthesise NAD+ in SAGM (sodium chloride, adenine, glucose, mannitol) phosphate solution and this synthesis was more in the presence of added nicotinic acid (optimum concentration 1 mM). The level of NAD+ decreased when the incubation period was increased from 24 to 48 hr. Glutamine had a deleterious effect on this synthesis, possibly due to the decrease in pH. Di-[2-ethyl hexyl] phthalate had an inhibitory effect on NAD+ synthesis when the cells were incubated in SAGM phosphate solution, either alone or in the presence of added nicotinic acid. There was significant decrease in the release of potassium and haemoglobin from the cells in the presence of nicotinic acid, indicating increased red cell stability. |
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Authors:
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A Santhosh; L R Lakshmi; P Arun; K V Deepadevi; K G Nair; V Manojkumar; P A Kurup |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Indian journal of biochemistry & biophysics Volume: 35 ISSN: 0301-1208 ISO Abbreviation: Indian J. Biochem. Biophys. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1999-01-25 Completed Date: 1999-01-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0310774 Medline TA: Indian J Biochem Biophys Country: INDIA |
Other Details:
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Languages: eng Pagination: 236-40 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, Peninsula Polymers Limited, Trivandrum, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine Adenosine Triphosphate / blood Blood Preservation Diethylhexyl Phthalate / pharmacology Erythrocytes / drug effects, metabolism* Glucose Humans Mannitol NAD / biosynthesis*, blood Niacin / metabolism, pharmacology Plasticizers / pharmacology Sodium Chloride |
| Chemical | |
Reg. No./Substance:
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0/ADSOL; 0/Plasticizers; 117-81-7/Diethylhexyl Phthalate; 50-99-7/Glucose; 53-84-9/NAD; 56-65-5/Adenosine Triphosphate; 59-67-6/Niacin; 69-65-8/Mannitol; 73-24-5/Adenine; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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