| Synthesis of glycerol nucleic acid (GNA) phosphoramidite monomers and oligonucleotide polymers. | |
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MedLine Citation:
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PMID: 20827716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This unit describes a straightforward method for preparing glycerol nucleic acid (GNA) phosphoramidite monomers and oligonucleotide polymers using standard cyanoethyl phosphoramidite chemistry. GNA is an unnatural nucleic acid analog composed of an acyclic three-carbon sugar-phosphate backbone that contains one stereogenic center per repeating unit. GNA has attracted significant attention as a nucleic acid derivative due to its unique ability to form stable Watson-Crick anti-parallel duplex structures with thermal and thermodynamic stabilities rivaling those of natural DNA and RNA. The chemical simplicity of this nucleic acid structure provides access to enantiomerically pure forms of right- and left-handed helical structures that can be used as unnatural building blocks in DNA nanotechnology. |
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Authors:
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Su Zhang; John C Chaput |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.] Volume: Chapter 4 ISSN: 1934-9289 ISO Abbreviation: Curr Protoc Nucleic Acid Chem Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-09 Completed Date: 2010-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101287865 Medline TA: Curr Protoc Nucleic Acid Chem Country: United States |
Other Details:
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Languages: eng Pagination: Unit4.40 Citation Subset: IM |
Copyright Information:
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(c) 2010 by John Wiley & Sons, Inc. |
Affiliation:
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Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Circular Dichroism DNA / chemistry* Glycerol / chemistry* Models, Molecular Nucleic Acids / chemistry* Oligonucleotides / chemistry* Organophosphorus Compounds / chemical synthesis*, chemistry* Polymers / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Nucleic Acids; 0/Oligonucleotides; 0/Organophosphorus Compounds; 0/Polymers; 0/phosphoramidite; 56-81-5/Glycerol; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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