| Synthesis of FAEEs from glycerol in engineered Saccharomyces cerevisiae using endogenously produced ethanol by heterologous expression of an unspecific bacterial acyltransferase. | |
| | |
MedLine Citation:
|
PMID: 21858787 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The high price of petroleum-based diesel fuel has led to the development of alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered to utilize glycerol as a substrate for ethanol production. For the synthesis of fatty acid ethyl esters (FAEEs) by engineered S. cerevisiae that utilize glycerol as substrate, heterologous expression of an unspecific acyltransferase from Acinetobacter baylyi with glycerol-utilizing genes was established. As a result, the engineered YPH499 (pGcyaDak, pGupWs-DgaTCas) strain produced 0.24 g/L FAEEs using endogenous ethanol produced from glycerol. And this study also demonstrated the possibility of increasing FAEE production by enhancing ethanol production by minimizing the synthesis of glycerol. The overall FAEE production in strain YPH499 fps1Δ gpd2Δ (pGcyaDak, pGupWs-DgaTCas) was 2.1-fold more than in YPH499 (pGcyaDak, pGupWs-DgaTCas), with approximately 0.52 g/L FAEEs produced, while nearly 17 g/L of glycerol was consumed. These results clearly indicated that FAEEs were synthesized in engineered S. cerevisiae by esterifying exogenous fatty acids with endogenously produced-ethanol from glycerol. This microbial system acts as a platform in applying metabolic engineering that allows the production of FAEEs from cheap and abundant substrates specifically glycerol through the use of endogenous bioethanol. Biotechnol. Bioeng. © 2011 Wiley Periodicals, Inc. |
| | |
Authors:
|
Kyung Ok Yu; Ju Jung; Seung Wook Kim; Chul Hwan Park; Sung Ok Han |
Related Documents
:
|
4204907 - Genetically controlled removal of "spore photoproduct" from deoxyribonucleic acid of ul... 22044447 - The substrate specificities of sunflower and soybean phospholipases d using transphosph... 3076177 - Studies on the anticandidal mode of action of allium sativum (garlic). 3916277 - Physiological characteristics of streptococcus dysgalactiae and streptococcus uberis an... 4083477 - A comparison of internal and external standardization in amino acid analysis. 10612067 - Silicic acid: its gastrointestinal uptake and urinary excretion in man and effects on a... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-8-19 |
Journal Detail:
|
Title: Biotechnology and bioengineering Volume: - ISSN: 1097-0290 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-8-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Wiley Periodicals, Inc. |
Affiliation:
|
School of Life Science and Biotechnology, Korea University, Seoul 136-701, Republic of Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Validation of long-term primary neuronal cultures and network activity through the integration of re...
Next Document: Putting cells under pressure: a simple and efficient way to enhance the productivity of medium-chain...