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Synthesis, DNA binding, and photonuclease activity of new tetraaza macrocyclic constrained isoxazole rings as subunit in metal complexes.
MedLine Citation:
PMID:  23145951     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The DNA-binding and photonuclease activity of newly synthesized tetra-azamacrocyclic ligand L (C(32)H(32)N(8)O(4)) and its complexes of type [MLCl(2)] and [ML]Cl(2) (where M = Co(II), Fe(II) and Cu(II); L = N,N'-[3-(4-{5-[(2-amino-ethylamino)-methyl]-isoxazol-3yl}-phenyl)-isoxazol-5-yl methyl-ethane-1,2-diamine] are specified. An octahedral geometry has been proposed for Fe(II) and Co(II) complexes, while the Cu(II) complex has a square planar environment. The absorption spectral results indicate that the complexes bind with the base pairs of DNA, with an intrinsic binding constant K(b) of Fe(II), Co(II), and Cu(II) complexes found to be 3.2 × 10(4) M(-1), 5.3 × 10(4) M(-1), and 4.2 × 10(4) M(-1), respectively, in 5 mM Tris-HCl/50 mM NaCl buffer at pH 7.2. The large enhancement in the relative viscosity of DNA on binding to the complexes supports the proposed DNA binding modes. The viscosity and thermal denaturation studies sustain the effective intercalation with DNA. The DNA photocleavage studies demonstrated that compounds exhibit significant photonuclease activity by a concentration dependent on singlet oxygen mediated mechanism.
Authors:
N Sharath; H S Bhojya Naik; B Vinay Kumar
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleosides, nucleotides & nucleic acids     Volume:  31     ISSN:  1532-2335     ISO Abbreviation:  Nucleosides Nucleotides Nucleic Acids     Publication Date:  2012  
Date Detail:
Created Date:  2012-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892832     Medline TA:  Nucleosides Nucleotides Nucleic Acids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  813-29     Citation Subset:  IM    
Affiliation:
a Department of Studies and Research in Industrial Chemistry, School of Chemical Sciences , Kuvempu University , Shankaraghatta , India.
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