| Synthesis and characterizations of new glycidyl-based cationic poly(aminoester) and study on gene delivery. | |
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MedLine Citation:
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PMID: 20420887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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New glycidyl-based (epoxide-based) poly(aminoester) (EPAE) containing hydroxyl and amino groups in the backbone and side chain was synthesized. EPAE self-assembled readily with the plasmid DNA(pCMV-betagal) in HEPES buffer and was characterized by dynamic light scattering, Zeta-potential, fluorescence images, and XTT cell viability assays. To evaluate the effect of molecular weight of EPAE system on transfection, EPAE polymers with three different molecular weights (EPAE22k, EPAE18k, and EPAE8k) were also prepared. This study found that all EPAE polymers were able to bind plasmid DNA and yielded positively charged complexes with a nano-sized particles (200 nm). The EPAE22k/DNA and EPAE18k/DNA complexes were able to transfect COS-7 cell in vitro with higher transfection efficiency than other EPAE8k/DNA. These results demonstrated that molecular weight of EPAE system had a significant effect on transferring ability. Examination of the cytotoxicity of PEI25k and EPAEs system revealed that EPAEs system had lower cytotoxicity. In this article, EPAEs seemed to be a novel cationic poly(aminoester) for gene delivery and an interesting candidate for further study. |
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Authors:
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Chao-Hsien Lin; Yu-Chih Hsiao; Min-Da Shau |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-24 |
Journal Detail:
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Title: International journal of pharmaceutics Volume: 393 ISSN: 1873-3476 ISO Abbreviation: Int J Pharm Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-01 Completed Date: 2010-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804127 Medline TA: Int J Pharm Country: Netherlands |
Other Details:
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Languages: eng Pagination: 135-42 Citation Subset: IM |
Copyright Information:
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2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Rd., Sec 1, Jen-Te, Taiwan, ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Buffers COS Cells Cell Survival / drug effects Cercopithecus aethiops Chromatography, Gel DNA / chemistry*, metabolism Dose-Response Relationship, Drug Epoxy Compounds / chemical synthesis*, toxicity Hela Cells Humans Hydrolysis Light Magnetic Resonance Spectroscopy Methacrylates / chemical synthesis*, toxicity Molecular Structure Molecular Weight Nanoparticles Plasmids* Polymers / chemical synthesis*, toxicity Scattering, Radiation Spectroscopy, Fourier Transform Infrared Time Factors Transfection / methods* |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Epoxy Compounds; 0/Methacrylates; 0/Polymers; 0/poly(beta-amino ester); 106-91-2/glycidyl methacrylate; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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