Document Detail

Synthesis and characterization of a hydrogel with controllable electroosmosis: a potential brain tissue surrogate for electrokinetic transport.
MedLine Citation:
PMID:  21905710     Owner:  NLM     Status:  MEDLINE    
Electroosmosis is the bulk fluid flow initiated by application of an electric field to an electrolyte solution in contact with immobile objects with a nonzero ζ-potential such as the surface of a porous medium. Electroosmosis may be used to assist analytical separations. Several gel-based systems with varying electroosmotic mobilities have been made in this context. A method was recently developed to determine the ζ-potential of organotypic hippocampal slice cultures (OHSC) as a representative model for normal brain tissue. The ζ-potential of the tissue is significant. However, determining the role of the ζ-potential in solute transport in tissue in an electric field is difficult because the tissue's ζ-potential cannot be altered. We hypothesized that mass transport properties, namely the ζ-potential and tortuosity, could be modulated by controlling the composition of a set of hydrogels. Thus, poly(acrylamide-co-acrylic acid) gels were prepared with three compositions (by monomer weight percent): acrylamide/acrylic acid 100/0, 90/10, and 75/25. The ζ-potentials of these gels at pH 7.4 are distinctly different, and in fact vary approximately linearly with the weight percent of acrylic acid. We discovered that the 25% acrylic acid gel is a respectable model for brain tissue, as its ζ-potential is comparable to the OHSC. This series of gels permits the experimental determination of the importance of electrokinetic properties in a particular experiment or protocol. Additionally, tortuosities were measured electrokinetically and by evaluating diffusion coefficients. Hydrogels with well-defined ζ-potential and tortuosity may find utility in biomaterials and analytical separations, and as a surrogate model for OHSC and living biological tissues.
Amir H Faraji; Jonathan J Cui; Yifat Guy; Ling Li; Colleen A Gavigan; Timothy G Strein; Stephen G Weber
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-10-20
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  27     ISSN:  1520-5827     ISO Abbreviation:  Langmuir     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-08     Completed Date:  2012-03-06     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13635-42     Citation Subset:  IM    
Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
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MeSH Terms
Acrylates / chemistry*
Hippocampus / metabolism*
Hydrogen-Ion Concentration
Grant Support
Reg. No./Substance:
0/Acrylates; 0/Hydrogels; J94PBK7X8S/acrylic acid

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