| Synthesis of [35S]thiophosphoryl adenylic acid, utilizing a general procedure for [35S]thiophosphoryl chloride production. | |
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MedLine Citation:
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PMID: 8465947 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Elemental [35S]sulfur was shown to equilibrate with the sulfur of thiophosphoryl chloride when these materials are heated together. This isotopic exchange reaction is the basis of a convenient, microscale synthesis of high specific activity [35S]PSCl3. [35S]Thiophosphoryl chloride is otherwise not commercially available except through custom synthesis. The labeled thiophosphoryl chloride was used in a novel procedure for the preparation of [35S]adenosine 5'-phosphorothioate. This isotopic exchange method should find wide application in the synthesis of many radiolabeled thiophosphoryl esters which utilize PSCl3 as the source of the thiophosphoryl group. |
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Authors:
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J T Slama; A M Simmons; T M Hernandez; R W Keenan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 209 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-05-03 Completed Date: 1993-05-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 143-9 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284-7760. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Monophosphate
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analogs & derivatives*,
chemical synthesis Chlorides / chemistry* Isotope Labeling / methods* Kinetics Phosphorus / chemistry* Phosphorus Compounds* Sulfur / chemistry Sulfur Radioisotopes / chemistry* Thionucleotides / chemical synthesis* |
| Grant Support | |
ID/Acronym/Agency:
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GM 32821/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Phosphorus Compounds; 0/Sulfur Radioisotopes; 0/Thionucleotides; 19341-57-2/adenosine 5'-phosphorothioate; 3982-91-0/thiophosphoryl chloride; 61-19-8/Adenosine Monophosphate; 7704-34-9/Sulfur; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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