Document Detail

Synthesis of 1,25-dihydroxyvitamin D3 by spleen cells isolated from two patients with myelofibrosis and a normal subject.
MedLine Citation:
PMID:  2308336     Owner:  NLM     Status:  MEDLINE    
The ability to synthesise [3H]1,25(OH)2D3 was studied in spleen cells incubated in short-term primary culture with [3H]25(OH)D3 which were isolated from two patients with idiopathic myelofibrosis and from one normal subject. Formation of a metabolite co-chromatographing with authentic 1,25(OH)2D3 on three different high-performance liquid chromatography systems was observed for cells from all three patients. [3H]1,25(OH)2D3 synthesis was 0.37 and 1.6 fmol/10(6) cells/incubation for cells with a density below that of lymphocyte separating media (1.077 g/ml) for the two myelofibrotic patients respectively and 0.15 fmol/10(6) cells/incubation for the normal subject. The most likely cell type capable of this synthesis were those of the monocyte-macrophage lineage which would have been present in abnormally high numbers in the patients with myelofibrosis. However, the exact identity of the cell-type responsible could not be determined because of the heterogeneity of cell types present. The observation that spleen cells from two patients with myelofibrosis and from a normal accident victim could metabolise [3H]25(OH)D3 to its active form [3H]1,25(OH)2D3 suggests a possible role for this metabolite in spleen haematopoiesis.
M E Hayes; M Davies; D Bayley; J A Lin Yin; E B Mawer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of steroid biochemistry     Volume:  35     ISSN:  0022-4731     ISO Abbreviation:  J. Steroid Biochem.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-04-06     Completed Date:  1990-04-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0260125     Medline TA:  J Steroid Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  191-4     Citation Subset:  IM    
Department of Medicine, Manchester University, England.
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MeSH Terms
Calcitriol / analysis,  biosynthesis*
Chromatography, High Pressure Liquid
Primary Myelofibrosis / metabolism*
Reference Values
Scintillation Counting
Spectrophotometry, Ultraviolet
Spleen / cytology,  metabolism*
Reg. No./Substance:

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