Document Detail

Synthesis of [1-11C], [2-11C], [1-11C](2H3) and [2-11C](2H3)acetate for in vivo studies of myocardium using PET.
MedLine Citation:
PMID:  9234364     Owner:  NLM     Status:  MEDLINE    
Four isotopically-labelled acetates ([1-11C], [2-11C], [1-11C](2H3) and [2-11C](2H3)acetate) were synthesized and used in positron emission tomography (PET) studies of pig myocardium. The [1-11C]acetates were synthesized by carboxylation of the appropriate 1H or 2H methyl Grignard reagents immobilized on a C2 solid phase extraction column (SPE). Purification by reverse-phase HPLC, resulted in 35-45% decay-corrected radiochemical yield with a total synthesis time of 25 min, and a radiochemical purity higher than 99%. The [2-11C]acetates were synthesized by carboxylation of 11C-labelled 1H or 2H methyl lithium. Purification as above resulted in 35-55% decay-corrected radiochemical yield with a total synthesis time of 30 min, and a radiochemical purity higher than 99%. Position-specific labelling was assessed by 13C-labelling and NMR. Multiple isotopic labelling by the combination of position-specific 11C-labelling and 2H substitution, has the potential to highlight different aspects of a complex biochemical system using a selected set of tracers in comparative PET studies. An illustration of this principle is given using acetate, where citric acid cycle metabolism results in a position-specific kinetic for the 11C-label, and deuteration opens up the possibility for the proton-abstracting processes within the citric acid cycle to be assessed.
T Kihlberg; S Valind; B Långström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nuclear medicine and biology     Volume:  21     ISSN:  0969-8051     ISO Abbreviation:  Nucl. Med. Biol.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1997-09-18     Completed Date:  1997-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9304420     Medline TA:  Nucl Med Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1067-72     Citation Subset:  IM    
Department of Organic Chemistry, Institute of Chemistry, Uppsala, Sweden.
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MeSH Terms
Acetates / chemical synthesis*,  diagnostic use,  isolation & purification
Carbon Radioisotopes / chemistry*
Chromatography, High Pressure Liquid
Deuterium / chemistry*
Heart / radionuclide imaging*
Tomography, Emission-Computed
Reg. No./Substance:
0/Acetates; 0/Carbon Radioisotopes; 7782-39-0/Deuterium

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