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Syntaxin-3 is required for melanosomal localization of Tyrp1 in melanocytes.
MedLine Citation:
PMID:  23549422     Owner:  NLM     Status:  Publisher    
Melanogenic enzymes are transported by vesicular/membrane trafficking to immature melanosomes in melanocytes where they catalyze the synthesis of melanin pigments. Although several factors involved in melanogenic enzyme trafficking have been identified in the past decade, involvement of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins, which generally mediate membrane fusion, on melanosomes in the process of melanogenic enzyme trafficking has never been investigated. In this study we identified syntaxin-3, which was originally described as a target SNARE protein at the plasma membrane, as a melanosome-resident protein and investigated whether syntaxin-3 is involved in the trafficking of the melanogenic enzyme Tyrp1 (tyrosinase-related protein 1) in mouse melanocytes. The results showed that knockdown of endogenous syntaxin-3 protein in melanocytes caused a dramatic reduction in Tyrp1 signals, especially from peripheral melanosomes, presumably as a result of lysosomal degradation of Tyrp1. They also showed that syntaxin-3 interacts with another target SNARE SNAP23 (synaptosome-associated protein of 23 kDa) and with vesicle SNARE VAMP7 (vesicle-associated membrane protein 7), which has been shown to be localized at Tyrp1-containing vesicles/organelles. These findings suggested that the SNARE machinery composed of VAMP7 on Tyrp1-containing vesicles and syntaxin-3 and SNAP23 on melanosomes regulates Tyrp1 trafficking to the melanosome in melanocytes.Journal of Investigative Dermatology accepted article preview online, 2 April 2013; doi:10.1038/jid.2013.156.
Ayaka Yatsu; Norihiko Ohbayashi; Kanako Tamura; Mitsunori Fukuda
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-4-02
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  -     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-4-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi Japan.
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