Document Detail


Synovial fluid RANKL and matrix metalloproteinase levels in enthesitis related arthritis subtype of juvenile idiopathic arthritis.
MedLine Citation:
PMID:  19052752     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In chronic arthritis cartilage and bone destruction occur as a consequence of synovial inflammation. It is mainly mediated by matrix metalloproteinases and RANKL-OPG pathways. Data on synovial fluid levels of these mediators in enthesitis related arthritis subtype (ERA) of JIA are not available. MMP-1, MMP-3, TIMP, sRANKL and OPG levels were measured in synovial fluid from patients with ERA and compared with other arthritides, polyarticular (Poly) JIA, RA and osteoarthritis (OA). sRANKL was detectable in 25/41 of ERA patients, 4/16 of Poly JIA patients. Median SF sRANKL level in patients with ERA was higher as compared to OA (p < 0.001) and poly JIA (p < 0.05) but were comparable to RA. The median OPG level in ERA was lower as compared to OA (p < 0.001), comparable to RA but was higher than poly JIA (p < 0.001). sRANKL/OPG ratio was significantly higher in ERA and Poly JIA compared to OA (p < 0.0001, p < 0.0001 respectively). The median MMP3 levels in ERA (74 microg/ml) was lower as compared to poly JIA (410 microg/ml; p < 0.0001) and RA (340 ug/ml; p < 0.0001) but was comparable to OA (107 microg/ml). The median level of ProMMP1 in ERA (0.70 microg/ml) was lower as compared to RA (2.9 microg/ml; p < 0.0001) and poly JIA but was elevated as compared to OA patients (0.1 microg/ml; p < 0.0001). TIMP1 levels in ERA were higher than poly JIA and RA patients. MMP3/TIMP1 ratio was lower in ERA compared to polyarticular JIA patients (p < 0.05). Ours is the first study reporting elevated sRANKL and reduced OPG levels and elevated sRANKL/OPG ratio in SF of children with JIA resulting in a mileu associated with bone loss. In addition, ERA patients had lower MMP level as well as MMP/TIMP ratio as compared to poly JIA which may partly explain lesser degree of joint damage seen in ERA as compared to poly JIA.
Authors:
Shilpi Agarwal; Ramnath Misra; Amita Aggarwal
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-04
Journal Detail:
Title:  Rheumatology international     Volume:  29     ISSN:  1437-160X     ISO Abbreviation:  Rheumatol. Int.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-29     Completed Date:  2009-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8206885     Medline TA:  Rheumatol Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  907-11     Citation Subset:  IM    
Affiliation:
Department of Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Arthritis, Juvenile Rheumatoid / classification*,  metabolism*
Arthritis, Rheumatoid / metabolism
Child
Child, Preschool
Female
Humans
Male
Matrix Metalloproteinases / metabolism*
Middle Aged
Osteoarthritis / metabolism
Osteoprotegerin / metabolism
RANK Ligand / metabolism*
Synovial Fluid / metabolism*
Tissue Inhibitor of Metalloproteinase-1 / metabolism
Chemical
Reg. No./Substance:
0/Osteoprotegerin; 0/RANK Ligand; 0/Tissue Inhibitor of Metalloproteinase-1; EC 3.4.24.-/Matrix Metalloproteinases

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