Document Detail


Synergy of photoacoustic and fluorescence flow cytometry of circulating cells with negative and positive contrasts.
MedLine Citation:
PMID:  22903924     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In vivo photoacoustic (PA) and fluorescence flow cytometry were previously applied separately using pulsed and continuous wave lasers respectively, and positive contrast detection mode only. This paper introduces a real-time integration of both techniques with positive and negative contrast modes using only pulsed lasers. Various applications of this new tool are summarized, including detection of liposomes loaded with Alexa-660 dye, red blood cells labeled with Indocyanine Green, B16F10 melanoma cells co-expressing melanin and green fluorescent protein (GFP), C8161-GFP melanoma cells targeted by magnetic nanoparticles, MTLn3 adenocarcinoma cells expressing novel near-infrared iRFP protein, and quantum dot-carbon nanotube conjugates. Negative contrast flow cytometry provided label-free detection of low absorbing or weakly fluorescent cells in blood absorption and autofluorescence background, respectively. The use of pulsed laser for time-resolved discrimination of objects with long fluorescence lifetime (e.g., quantum dots) from shorter autofluorescence background (e.g., blood plasma) is also highlighted in this paper. The supplementary nature of PA and fluorescence detection increased the versatility of the integrated method for simultaneous detection of probes and cells having various absorbing and fluorescent properties, and provided verification of PA data using a more established fluorescence based technique. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
Authors:
Dmitry A Nedosekin; Mustafa Sarimollaoglu; Ekaterina I Galanzha; Rupa Sawant; Vladimir P Torchilin; Vladislav V Verkhusha; Jie Ma; Markus H Frank; Alexandru S Biris; Vladimir P Zharov
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-20
Journal Detail:
Title:  Journal of biophotonics     Volume:  -     ISSN:  1864-0648     ISO Abbreviation:  J Biophotonics     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318567     Medline TA:  J Biophotonics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Arkansas Nanomedicine Center, Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA. DNedosekin@uams.edu.
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