Document Detail

Synergy between L-selectin signaling and chemotactic activation during neutrophil adhesion and transmigration.
MedLine Citation:
PMID:  9379058     Owner:  NLM     Status:  MEDLINE    
L-selectin enables capture and rolling of neutrophils on inflamed endothelium. This may facilitate the binding of agonists such as IL-8 and platelet-activating factor (PAF), which signal CD18-mediated firm adhesion and transmigration. Recent studies demonstrate that L-selectin can mediate transmembrane signaling. However, the functional effects of costimulation through agonist and L-selectin require further study. Here, we quantify cell adhesion, motility, and transmigration in response to co-activation through L-selectin and agonist. The surface expression of CD11b/CD18 increased and L-selectin decreased in proportion to the extent of L-selectin cross-linking. A flow cytometric assay was used to measure CD11b/CD18-dependent adhesion to fluorescent beads adsorbed with albumin. Neutrophil adhesion was detected within seconds of adding PAF (20 pM), IL-8 (50 pM), or cross-linking L-selectin. Costimulation through agonist and L-selectin potentiated by up to threefold the rate and extent of bead capture. Stimulation through L-selectin induced membrane ruffling, whereas PAF or IL-8 induced bipolar shape change. L-selectin cross-linking sustained the transient shape change induced by low concentrations (10-50 pM) of agonist. Chemokinesis stimulated by IL-8 was inhibited in the presence of cross-linking L-selectin. This was attributed to enhanced cell spreading following costimulation. Migration across HUVEC monolayers stimulated with IL-1 was also potentiated in the presence of L-selectin cross-linking. We propose that cross-linking of L-selectin and binding of agonist receptors may act synergistically to amplify neutrophil activation and emigration in the inflamed vasculature.
Y T Tsang; S Neelamegham; Y Hu; E L Berg; A R Burns; C W Smith; S I Simon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  159     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-11-12     Completed Date:  1997-11-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4566-77     Citation Subset:  AIM; IM    
Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030-2399, USA.
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MeSH Terms
Cell Adhesion / physiology
Cell Movement / physiology*
Chemotaxis / physiology*
L-Selectin / physiology*
Neutrophil Activation*
Neutrophils / cytology,  physiology*
Second Messenger Systems
Signal Transduction*
Grant Support
Reg. No./Substance:

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