| Synergistic effects of protein tyrosine kinase inhibitor genistein with camptothecins against three cell lines in vitro. | |
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MedLine Citation:
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PMID: 15893418 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Genistein, a natural isoflavone product has been shown to induce cellular death and increase the apoptotic cell death induced by several DNA-damaging stimuli. We have explored the combined effect of genistein and camptothecins against three cell lines, HeLa (cervical cancer), OAW-42 (ovarian cancer) and L929 (normal fibroblasts). Combined effect was estimated in 96-well plates using the SRB method and median-effect analysis. Addition of genistein synergistically increased the antiproliferative affect of camptothecins, inhibiting the camptothecin-induced G2/M arrest and increasing the apoptotic cell population. In HeLa cells, genistein inhibited CDK1 phosphorylation after irinotecan treatment. Thus, abrogation of the G2/M checkpoint control by genistein may be a useful maneuver to increase cytotoxicity of agents that damage DNA and inhibit cell-cycle progression in the G2/M boundary. |
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Authors:
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Konstantinos T Papazisis; Theodora G Kalemi; Dimitra Zambouli; George D Geromichalos; Alexandros F Lambropoulos; Alexandros Kotsis; Lazaros L Boutis; Alexandros H Kortsaris |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Cancer letters Volume: 233 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-03-07 Completed Date: 2006-04-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 255-64 Citation Subset: IM |
Affiliation:
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Theagenion Cancer Institute, and Department of Biology, Medical Faculty, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece. konstantinos.papazisis@cancer.org.uk |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use* Apoptosis / drug effects CDC2 Protein Kinase / metabolism Camptothecin / administration & dosage Cell Division / drug effects Cell Proliferation / drug effects Cells, Cultured Drug Synergism Female Fibroblasts / cytology, drug effects*, enzymology G2 Phase / drug effects Genistein / administration & dosage Hela Cells / cytology, drug effects, enzymology Humans Ovarian Neoplasms / drug therapy*, enzymology, pathology Phosphorylation / drug effects Protein-Tyrosine Kinases / antagonists & inhibitors* |
| Chemical | |
Reg. No./Substance:
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446-72-0/Genistein; 7689-03-4/Camptothecin; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.11.22/CDC2 Protein Kinase |
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