| Synergistic effects on pregnancy-termination activity of DL111-IT in combination with mifepristone. | |
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MedLine Citation:
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PMID: 9812725 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To clarify the role of DL111-IT when combined with mifepristone (Mif) on termination of early pregnancy. METHODS: Progesterone receptors (PR) was measured using radioligand assay (RA), and peroxidase-antiperoxidase (PAP) immuno-histochemistry. Decidual cells (DC) were estimated using cell culture and histological examination (HE) (including fetus). RESULTS: DL111-IT 100 mg.kg-1 i.m., Mif 5 mg.kg-1 i.g., DL111-IT 16 mg.kg-1 i.m. + Mif 1.5 mg.kg-1 i.g. and tea seed oil (TSO) 2 mL.kg-1 i.m. on d 7 of pregnancy in rats, DC and fetus of treated groups were found to be degenerated at 24 h after treatment, at 48 h after treatment, PAP labeling index (%) of uterus PR of 4 groups were 22 +/- 4, 18.7 +/- 2.9, 10.3 +/- 1.2, 52 +/- 15, respectively. Rats i.m. DL111-IT 100 mg.kg-1 24 h after treatment, the quantity of PR decreased by 47% vs that of TSO. The affinity of PR with Mif and progesterone did not change. Cultured human DC were exposed to DL111-IT and Mif 0-50 mg.L-1, alone or combinatively, for 24 h. LD50 (mg.L-1) were: DL111-IT 18.1 (15.1-21.4), Mif 25.0 (23.1-26.9), DL111-IT 5.0 plus Mif 3.5 (1.8-6.5) or Mif 5.0 plus DL111-IT 4.6 (1.1-7.3), respectively. CONCLUSION: DL111-IT enhanced action of Mif on DC, reduced quantity of PR, so the 2 drugs had the synergistic action in termination of early pregnancy. |
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Authors:
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B Yang; R Y Fang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zhongguo yao li xue bao = Acta pharmacologica Sinica Volume: 17 ISSN: 0253-9756 ISO Abbreviation: Zhongguo Yao Li Xue Bao Publication Date: 1996 Jul |
Date Detail:
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Created Date: 1999-02-16 Completed Date: 1999-02-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8100330 Medline TA: Zhongguo Yao Li Xue Bao Country: CHINA |
Other Details:
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Languages: eng Pagination: 361-5 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Pharmacy, Zhejiang Medical University, Hangzhou, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abortifacient Agents, Nonsteroidal
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pharmacology Abortifacient Agents, Steroidal / pharmacology Abortion, Induced* Animals Cells, Cultured Decidua / cytology, drug effects Drug Synergism Female Humans Mifepristone / pharmacology* Pregnancy Rats Rats, Sprague-Dawley Receptors, Progesterone / metabolism Triazoles / pharmacology* Uterus / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Abortifacient Agents, Nonsteroidal; 0/Abortifacient Agents, Steroidal; 0/Receptors, Progesterone; 0/Triazoles; 69095-83-6/3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole; 84371-65-3/Mifepristone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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