| Synergistic effects of photodynamic therapy with hpph and gemcitabine in pancreatic cancer cell lines. | |
| | |
MedLine Citation:
|
PMID: 23018618 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND AND OBJECTIVE: Photodynamic therapy (PDT) is a potential treatment for pancreatic cancer. A second-generation photosensitizer, 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide (HPPH) has a long wavelength absorption, high-tumor selectivity, and shorter duration of skin photosensitivity. We investigated the efficacy of PDT with HPPH and gemcitabine in inducing cell death in multiple pancreatic cancer cell lines. METHODS: We used three pancreatic cancer cell lines (PANC-1, MIA PaCa-2, and BXPC-3) incubated with HPPH concentration of 0, 0.005, 0.01, 0.025, 0.05, 0.1, 0.25, and 0.5 µg/ml for 6 hours, followed by photoradiation at a light dose of 60 J/cm(2) . Afterwards, each cell line was treated with gemcitabine at concentrations of 0, 1, 10, and 100 µM and incubated for another 96 hours. Cell death was detected with SYTOX green staining. We also assessed the difference in cytotoxicity in adding gemcitabine before and after PDT. RESULTS: HPPH-PDT can effectively induce cell death in all cell lines in a dose-dependent manner, with a 100% of cell death at the 0.5 µg/ml HPPH concentration. In contrast, monotherapy with gemcitabine alone (100 µM) only achieved <45% cell death. Combining gemcitabine to HPPH-PDT resulted in synergistic cytotoxic effect with 20-50% more cell death across all cell lines. There was no difference in cytotoxicity in adding gemcitabine before or after PDT. CONCLUSION: This is the first study on HPPH-PDT for pancreatic cancer. HPPH-PDT-induced cell death occurs in a dose-dependent manner. HPPH-PDT and gemcitabine have synergistic effects in inducing cell death in multiple pancreatic cancer cell lines. Lasers Surg. Med. © 2012 Wiley Periodicals, Inc. |
| | |
Authors:
|
Gang Sun; Marlys A Anderson; Emmanuel C Gorospe; Cadman L Leggett; Lori S Lutzke; Louis M Wong Kee Song; Michael Levy; Kenneth K Wang |
Related Documents
:
|
22761038 - The molecular circuitry underlying pluripotency in embryonic stem cells. 23027898 - Cell biology: at the center of modern biomedicine. 22696478 - Sirt1, p53 and p38(mapk) are crucial regulators of detrimental phenotypes of escs with ... 23213208 - Possible role of pineal allopregnanolone in purkinje cell survival. 22014028 - Sensory epithelial cells acquire features of prosensory cells via epithelial to mesench... 17112618 - Uptake and metabolism of novel biodegradable poly (glycerol-adipate) nanoparticles in d... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-9-27 |
Journal Detail:
|
Title: Lasers in surgery and medicine Volume: - ISSN: 1096-9101 ISO Abbreviation: Lasers Surg Med Publication Date: 2012 Sep |
Date Detail:
|
Created Date: 2012-9-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8007168 Medline TA: Lasers Surg Med Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Division of Gastroenterology and Hepatology, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905; Department of Gastroenterology and Hepatology, Chinese PLA General Hospital, 28 Fu Xing Road, Beijing 100853, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Star-related lipid transfer domain protein 5 (STARD5) binds primary bile acids.
Next Document: Rapid fabrication of nanostructured surfaces using nanocoining.