Document Detail


Synergistic effects of interleukin-4 or interleukin-13 and tumor necrosis factor-alpha on eosinophil activation in vitro.
MedLine Citation:
PMID:  10030846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increased concentrations of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-4, and IL-13 have been measured in bronchoalveolar lavage fluid (BALF) of patients with asthma following allergen provocation. In addition, these cytokines have also been reported to activate eosinophils in vitro. Although cytokine interactions have been postulated in the activation of eosinophils, the combined effects of cytokines on eosinophil activation remain poorly understood. Because activation of eosinophils has been regarded as a crucial event in the pathogenesis of asthmatic inflammation, we tested the hypothesis that IL-4 and IL-13 could enhance the effects of TNF-alpha on eosinophil activation. For this purpose, eosinophils from normal donors were purified and cultured in the presence of IL-4 or IL-13 and TNF-alpha. Eosinophil survival and surface expression of CD69 were assessed by flow cytometry. There was a concentration- and time-dependent upregulation in CD69 expression as well as eosinophil survival when eosinophils were incubated with IL-13, IL-4, or TNF-alpha. However, eosinophil viability and CD69 expression increased synergistically when eosinophils were incubated with IL-13 or IL-4 in the presence of TNF-alpha. This synergistic effect of IL-4 and IL-13 on CD69 expression was not limited to TNF-alpha but was also observed with IL-5. Our study provides evidence that IL-4 can activate eosinophils in a similar fashion as does IL-13. Furthermore, this study shows that the addition of IL-4 or IL-13 to TNF-alpha or IL-5 has synergistic effects on eosinophil activation, suggesting that the combined effects of different cytokines present in BALF following allergen provocation can enhance eosinophil activation in vitro.
Authors:
W Luttmann; T Matthiesen; H Matthys; J C Virchow
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  20     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-02     Completed Date:  1999-04-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  474-80     Citation Subset:  IM    
Affiliation:
Department of Pneumology, Medical University Clinics, Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis
Antigens, Differentiation, T-Lymphocyte / analysis
Cell Survival / drug effects
Dose-Response Relationship, Drug
Drug Synergism
Eosinophils / drug effects*
Humans
Interleukin-13 / pharmacology*
Interleukin-4 / pharmacology*
Tumor Necrosis Factor-alpha / pharmacology*
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, T-Lymphocyte; 0/CD69 antigen; 0/Interleukin-13; 0/Tumor Necrosis Factor-alpha; 207137-56-2/Interleukin-4

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