Document Detail

Synergistic effects of food colors on the toxicity of 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) in primary cultured rat hepatocytes.
MedLine Citation:
PMID:  10955279     Owner:  NLM     Status:  MEDLINE    
The synergistic effect of food additives or food colors on the toxicity of 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) was investigated using primary cultured rat hepatocytes. When hepatocytes from rats fed a standard diet were treated with a mixture of four major food additives (sorbitol, sodium L(+)-glutamate, benzoic acid, and propylene glycol) or a mixture of six typical artificial food colors (erythrosine, allura red, new coccine, brilliant blue, tertrazine, and fast green), the in vitro treated food-color mixture itself showed cytotoxicity: the reduction of cell viability and decreases in the activities of gluconeogenesis and ureogenesis. The food-color mixture enhanced cytotoxicity of Trp-P-1 obviously. We then investigated the effects of in vivo-dosed food additives or food colors on Trp-P-1-caused toxicity. Hepatocytes were isolated and cultured from rats fed a diet containing a mixture of food additives or a mixture of food colors with half the amount of their respective acceptable daily intake for 4 wk. Trp-P-1 was administered to the hepatocytes at various concentrations for 12 h. Synergistic effects of in vivo-dosed food additives and food colors were not observed on Trp-P-1-caused cytotoxicity as estimated by a loss of cell viability and the reductions of DNA and protein syntheses. On the contrary, we have observed that in vivo administered food colors synergistically facilitated to reduce the activities of gluconeogenesis and ureogenesis in Trp-P-1-treated hepatocytes. These results suggest that the daily intake of artificial food colors may impair hepatic functions such as gluconeogenesis and ureogenesis, when dietary carcinogens are exposed to the liver cells.
H Ashida; T Hashimoto; S Tsuji; K Kanazawa; G Danno
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of nutritional science and vitaminology     Volume:  46     ISSN:  0301-4800     ISO Abbreviation:  J. Nutr. Sci. Vitaminol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-11-29     Completed Date:  2001-03-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0402640     Medline TA:  J Nutr Sci Vitaminol (Tokyo)     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  130-6     Citation Subset:  IM    
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan.
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MeSH Terms
Carbolines / toxicity*
Cell Survival / drug effects
Cells, Cultured
Cytotoxins / toxicity*
Drug Synergism
Food Additives / pharmacology*
Food Coloring Agents / pharmacology*
Gluconeogenesis / drug effects
Hepatocytes / drug effects*,  metabolism
Microsomes, Liver / drug effects,  enzymology
Random Allocation
Rats, Wistar
Urea / metabolism
Reg. No./Substance:
0/Carbolines; 0/Cytotoxins; 0/Food Additives; 0/Food Coloring Agents; 57-13-6/Urea; 62450-06-0/Trp-P-1

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