Document Detail


Synergistic effect with silver nanoparticles and its involved mechanisms.
MedLine Citation:
PMID:  22956753     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Silver nanoparticles (nano-Ag), which have well-known antimicrobial properties, are extensively used in various medical and general applications. In this study, the combination effects between nano-Ag and conventional antibiotics including ampicillin, chloramphenicol, and kanamycin against various pathogenic bacteria were investigated. We have tested MIC and fractional inhibitory concentration index (FICI) to confirm antibacterial susceptibility and synergistic effects. The results showed that nano-Ag possessed antibacterial effects and synergistic activities. Furthermore, the antibiofilm activities of nano-Ag alone or in combination with antibiotics were investigated. The formation of biofilm is associated with resistance to antimicrobial agents and chronic bacterial infections. The results indicated that nano-Ag also had antibiofilm activities. To understand these effects of nano-Ag, we conducted the ATPase inhibitor assay, permeability assay, and hydroxyl radicals assay. The antibacterial activity of nano-Ag was influenced by ATP-associated metabolism rather than permeability of outer membrane. Additionally, nano-Ag generated hydroxyl radicals which are a highly reactive oxygen species (ROS) induced by bactericidal agents. Therefore, we conclude that nano-Ag have potential as a combination therapeutic agent for treatment of infectious diseases by bacteria.
Authors:
In-Sok Hwang; Ji Hong Hwang; Hyemin Choi; Keuk-Jun Kim; Dong Gun Lee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-6
Journal Detail:
Title:  Journal of medical microbiology     Volume:  -     ISSN:  1473-5644     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Kyungpook National University.
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