Document Detail

Synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens.
MedLine Citation:
PMID:  1324304     Owner:  NLM     Status:  MEDLINE    
Our previous studies have shown that pyrophosphate (PPi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. The purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of PPi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (PPi-SDS) inhibit growth synergistically. Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Eikenella corrodens, and Campylobacter rectus (formerly Wolinella recta) were cultured in appropriate enriched media under anaerobic conditions. Inhibition assays were performed in tubes containing media supplemented with PPi and/or SDS. A range of concentrations of PPi and SDS in 2-fold increments was employed, with each concentration assayed in triplicate. Minimal inhibitory concentration (MIC) analyses revealed all of the bacteria were susceptible to PPi and SDS, with MICs of 0.67% (25 mM) and 0.01% w/v respectively. Combination studies with PPi-SDS showed much greater growth inhibition against P. gingivalis and A. actinomycetemcomitans than achieved with the agents individually. Determination of fractional inhibitory concentration indices indicated a synergistic growth-inhibitory effect. Under the constraints of the conditions employed, these studies demonstrate the efficacy of PPi-SDS combinations in inhibiting the growth of periodontal pathogens. It is conceivable that these compounds may have clinical benefit as a subgingival irrigant.
D R Drake; J Chung; W Grigsby; C Wu-Yuan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of periodontology     Volume:  63     ISSN:  0022-3492     ISO Abbreviation:  J. Periodontol.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-09-24     Completed Date:  1992-09-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  696-700     Citation Subset:  D; IM    
Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City.
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MeSH Terms
Actinobacillus actinomycetemcomitans / drug effects,  growth & development
Bacteria / drug effects*,  growth & development
Campylobacter / drug effects,  growth & development
Colony Count, Microbial
Diphosphates / administration & dosage,  pharmacology*
Dose-Response Relationship, Drug
Drug Synergism
Eikenella corrodens / drug effects,  growth & development
Fusobacterium nucleatum / drug effects,  growth & development
Periodontal Diseases / microbiology*
Porphyromonas gingivalis / drug effects,  growth & development
Sodium Dodecyl Sulfate / administration & dosage,  pharmacology*
Reg. No./Substance:
0/Diphosphates; 151-21-3/Sodium Dodecyl Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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