Document Detail

Synergistic effect of interleukin-1alpha and ceramide analogue on production of prostaglandin E2 and F2alpha by endometrial stromal cells.
MedLine Citation:
PMID:  11775008     Owner:  NLM     Status:  MEDLINE    
PROBLEM: Prostaglandins (PGs) are synthesized in the endometrium. Our objective was to evaluate interleukin (IL)-1alpha-induced production of PGE2 and PGF2alpha in endometrial stromal cells (ESC) following treatment with ceramide analogues. METHODS OF STUDY: ESC were obtained from human uterine endometrium by enzymic digestion and filtration. ESC were treated with IL-1alpha, IL-1 receptor antagonist (ra), C2-ceramide and C6-ceramide. The concentrations of PGE2 and PGF2alpha in media were determined using ELISA. The induction of prostaglandin H synthase (PGHS)-2 mRNA was also ascertained by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The production of PGE2 and PGF2alpha was significantly increased by IL-lalpha and suppressed by IL-1 ra, in a dose-dependent manner. PGF2alpha production was further increased by treatment with the combination of IL-1alpha and C2-ceramide as compared with IL-1alpha treatment alone. There was no significant difference in PGE2 production between cells treated with IL-1alpha and C2-ceramide and those treated with IL-1alpha alone. Both PGE2 and PGF2alpha production were significantly increased by treatment with IL-1alpha and C6-ceramide as compared with IL-1alpha treatment alone. Treatment of ESC with IL-1alpha stimulated PGHS-2 mRNA. PGHS-2 mRNA was decreased when IL-1 ra was added to the IL-1alpha-stimulated cells. CONCLUSIONS: These results suggest that IL-1alpha stimulates the production of PGE2 and PGF2alpha by a mechanism that involves the sphingomyelin-ceramide system, and thus that ceramide may be important in increasing the production of PGE2 and PGF2alpha in the human endometrium.
Y Kawano; S Nakamura; J Fukuda; I Miyakawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  46     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-31     Completed Date:  2002-07-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  393-8     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Oita Medical University, Hasama-machi, Japan.
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MeSH Terms
Cells, Cultured
Ceramides / pharmacology*
Dinoprost / biosynthesis*
Dinoprostone / biosynthesis*
Drug Synergism
Endometrium / cytology,  immunology*
Interleukin 1 Receptor Antagonist Protein
Interleukin-1 / pharmacology*
Prostaglandin-Endoperoxide Synthases / genetics
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins / pharmacology
Sphingosine / analogs & derivatives*,  pharmacology*
Stromal Cells / cytology,  drug effects,  metabolism
Reg. No./Substance:
0/Ceramides; 0/IL1RN protein, human; 0/Interleukin 1 Receptor Antagonist Protein; 0/Interleukin-1; 0/N-acetylsphingosine; 0/N-caproylsphingosine; 0/RNA, Messenger; 0/Sialoglycoproteins; 123-78-4/Sphingosine; 363-24-6/Dinoprostone; 551-11-1/Dinoprost; EC Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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